livestock-nutrition-2016_posters-accepted-abstracts

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Volume 4, Issue 2 J Fisheries Livest Prod
ISSN: 2332-2608 JFLP, an open access journal
Page 53
Livestock Nutrition 2016
July 21-22, 2016
conferenceseries.com
July 21-22, 2016 Brisbane, Australia
2nd International Conference on
Livestock Nutrition
Neutrophilic toll-like receptor 4, Fas gene expression and level of some plasma parameters: Causative factor for
repeat breeding
Anand Laxmi N, Dandage Shashikant Damodhar and Amit Kumar
National Dairy Research Institute, India
Insulin like growth factor-1 (IGF-1) plays an important role in reproduction because it has anabolic and positive effect on cell proliferation,
transformation and differentiation. IGF-1 also plays a prominent role in the regulation of immunity and inflammation. Plasma lactoferrin
(LF) and haptoglobin (Hp) reflect the immune status of the animal. Higher level of plasma Hp indicates sub-clinical or clinical infections. The
present study was conducted in order to investigate whether the TLR-4 and Fas gene expression in neutrophils and plasma level of IGF 1, LF and
Hp varied between repeat breeding (RB) and regular breeding (RgB) cross bred cattle. For the study, recently calved Karan Fries cattle of 2nd-3rd
parity with body weight ranging from 400-460 kg reared under farm and field conditions and free from clinical reproductive tract infections
and mastitic conditions were selected. Cows were monitored up to three consecutive services. Animals which conceived by maximum three
number of services (23 weeks post partum) were considered as regular breeders and those that did not conceive, as repeat breeders. Relative
expression of TLR-4 and Fas genes in neutrophils were significantly (P<0.05) higher in RgB group when compared with RB group. On in
vitro supplementation of IGF-1, the relative expression of TLR-4 and Fas gene in neutrophils of RgB group increased but was not significant.
Concentration of plasma IGF-1 and LF were significantly (P<0.001) greater in RgB group while plasma Hp was significantly (P<0.001) less in
RgB group when compared with RB group. Within RgB group the concentration of IGF-1 significantly increased post 9th week but such an
increase was not observed in RB group. The concentration of plasma Hp at the beginning, at the end of 23 weeks of experiment and also at
weekly interval was significantly greater in RB group when compared with RgB group. The concentration of LF increased significantly in RgB
group from 12th week postpartum and was significantly higher in RB group. IGF-1 and LF parameters were positively correlated with each other
and both of them were negatively correlated with Hp. From the present study, it can be concluded that the immune status of RgB group animals
was better when compared with RB group animals. Poor immune status and some type of inflammation may be the causative factor for repeat
breeding problem to persist in one of the group of cows.
J Fisheries Livest Prod 2016, 4:2 (Suppl)
Evaluation of a short-horn grasshopper Spathosternumpraciniferum as an alternative protein source for livestock
feed
Arijit Gangopadhyay1 and Parimalendu Haldar2
1Achhruram Memorial College, India
2Visva-Bharati University, India
Despite being a capable alternative protein source in livestock diets, grasshoppers received fewer attentions regarding mass scale cultivation.
Probable annual biomass of two short-horn grasshoppers of the genus Oxya has been estimated so far but both the works used an
environmental chamber, the cost of which is a definite encumbrance for local livestock farmers. Hence it is essential to estimate the annual
biomass in natural condition. In this context the present work explores whether the short-horn grasshopper Spathosternum praciniferum could
be a suitable alternative protein supplement. Firstly, the proximate composition along with fatty acids, amino acids, vitamins, minerals and
anti-nutritional factors were estimated. Then they were reared in the laboratory with the host plant Sorghum halepense and nymphal mortality
was calculated followed by estimation of sex ratio. Number of egg pods laid by each female and number of eggs hatched were also determined.
Results revealed that the insects are highly nutritious with more than 65% protein and are affluent in essential amino acids. Total six fatty acids
and five vitamins were detected. Anti-nutrients were also present in extremely low titer. The results have instated these insects as a rich nutrient
resource; moreover, the projected annual biomass was found to be about 3.5 kg of dry weight obtained from a single pair. This is certainly lower
than the earlier biomass estimations, but one should note that environmental chamber was not used in the present work. Hence the present
findings strongly established the candidature of short-horned grasshoppers as an alternative protein source for livestock.