livestock-nutrition-2016_posters-accepted-abstracts

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Volume 4, Issue 2 J Fisheries Livest Prod
ISSN: 2332-2608 JFLP, an open access journal
Page 59
Livestock Nutrition 2016
July 21-22, 2016
conferenceseries.com
July 21-22, 2016 Brisbane, Australia
2nd International Conference on
Livestock Nutrition
Changes in metabolic and hormonal profiles with heat shock protein-70 and their association with
follicular dynamics in dairy cattle in the subtropical region of Peshawar
Muhammad Subhan Qureshi, Ikramullah Khan, Sohail Akhtar, Ijaz Ali and Ghufranullah
University of Agricultural Peshawar, Pakistan
Thermal stress affects the fertility of high producing dairy cattle, badly. This study was conducted to compare Holstein Frisian
(HF), cross-bred and indigenous dairy cattle (Sahiwal and Achai) in terms of effects of ambient temperature on HSP-70
expression, serum P4, glucose and cortisol concentrations and follicular number during the dioestrus period of the estrous cycle.
Thirty six multiparous lactating dairy cows with normal cyclicity comprising nine cows from each breed of Sahiwal, Achai, cross-
bred and HF were selected in two state farms in Peshawar located at 34.0°N, 71.6°E. Blood sampling was conducted at an average
ambient temperature of 18°C (thermoneutral) in February, 32°C (thermal transitional) in April and 42°C (thermal stress) in late
June. Rectal ovarian ultrasonography was performed to determine follicular dynamics. Thermal stress increased concentrations of
glucose (P<0.05), cortisol (P<0.001), HSP-70 (P<0.001) and number of follicles (P<0.01), while decreased progesterone (P<0.01) in
all four breeds beyond 32°C; however, Achai cows resisted elevation of HSP-70 levels with the increasing ambient temperature up to
32°C. As the ambient temperature increased to 32°C and 42 °C, a notable elevation was observed in the first four parameters while
progesterone concentrations were decreased. Positive correlation was found between level of glucose, cortisol and HSP-70 with the rise
in ambient temperature while progesterone concentrations related negatively with the changing temperature. The number of follicles
related positively with ambient temperature, cortisol, HSP-70 and blood glucose and negatively with progesterone concentrations.
It is concluded that thermal stress elevated concentration of HSP-70 associated with enhanced concentration of glucose, cortisol
and number of follicles. Progesterone concentration provided a good indication of fertility and related negatively with the thermal
stress; however, the number of follicles may be considered as a negative indicator of fertility due to lack of a dominant follicle. The
indigenous dairy breeds resisted heat stress better than the exotic and crossbred cows indicating that the later are more susceptible to
heat stress than the former breeds.
J Fisheries Livest Prod 2016, 4:2 (Suppl)
A study to estimate longevity of thermostable Newcastle disease vaccine (strain I-2) in village chicken
of Nepal
Shankar Nyaupane1, B B Pokharel2 and M P Acharya 3
1Tribhuvan University, Nepal
2University of Guelph, Canada
3Nepal Agriculture Research Council, Nepal
Newcastle disease (ND) is one of the most important poultry diseases because of its widespread distribution and economic impact
on poultry. The present study was conducted to estimate the longevity of thermostable ND vaccine (NDV strain I-2) in village
chickens of Nepal. A total of 56 (27 days old chicks) were allocated randomly into 2 groups (treatment and control) with 28 birds in
each group. On day 28, ND vaccine was administered to the treatment group only. Blood sample was collected from experimental
birds at 1 day prior to vaccination and 14, 21, 30, 60, 90 and 105 days post vaccination. The serum obtained was titrated for NDV
antibody using hemagglutination inhibition test. The data obtained were log transformed and subjected to the SPSS 16.0. The antibody
response of treatment group revealed that the log2HI titers were 5.7, 7.0, 7.2, 6.0, 3.7 and 3.1 for 14, 21, 30, 60, 90 and 105 days after
vaccination. The treatment group had higher (P<0.05) antibody titer level at 14, 21, 30, 60 and 90 days after vaccination compared
to control group however, there was no significant difference in the mean titer level between treatment and control group on 105
days after vaccination suggesting that booster dose is required after 90 days of primary vaccination. Thus, thermostable ND vaccine
(strain I-2) produced specific immunity against ND for at least 90 days after vaccination and may be considered suitable in Nepalese
condition where cold chain maintenance is a huge challenge especially in rural area.