livestock-nutrition-2016_posters-accepted-abstracts

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Volume 4, Issue 2 J Fisheries Livest Prod
ISSN: 2332-2608 JFLP, an open access journal
Page 55
Livestock Nutrition 2016
July 21-22, 2016
conferenceseries.com
July 21-22, 2016 Brisbane, Australia
2nd International Conference on
Livestock Nutrition
Influence of SNPs in myostatin promoter gene to growth and muscling traits in Bali cattle
Himmatul Khasanah
Bogor Agricultural University, Indonesia
Improvement of genetic qualities in Bali cattle can be done by selection based on genetic marker. MSTN (Myostatin) gene is known
as an inhibitor or negative regulator of muscles development in embryogenesis andmyogenesis. Mutation of this gene caused double
muscling characteristic in cattle and it was identified has significant associated with some growth traits in cattle, sheep, pigs and mice.
A total 48 Bali cattle from Bali cattle breeding centre was screened to identified genetic polymorphisms in MSTN promoter region
by using direct-sequencing method (GenBank: AF348479.1). The weights and bodies measurement of Bali cattle were collected at
12 months. The muscling traits were evaluated by using ultrasound veterinary scanner at frequency 6.5 Hz and 130 mm of deep. The
polymorphic SNPs in this region were 23 SNPs (g.-8495C>T, g.-8455A>C, g.-8444G>A, g.-8428A>G, g.8361G>A, g.-8350A>G, g.-
8313A>G, g.-8254T>G, g.-8223C>T, g.-8313A>G, g.-8.184C>A, g.-8173A>G, g.-8161C>T, g.-8158C>A, g.-8144T>C, g.-8141G>C,
g.-8124T>C, g.-8098C>T, g.-8087C>G, g.-8086C>T. Based on statistical analyses, SNPs g.-8444G>A, g.-8428A>G, g.8361G>A, g.-
8313A>G and g.-8313A>G were significantly associated with ultrasound back fat thickness. SNP g.-8.184C>A and g.-8087C>G was
significantly associated with ultrasound rump thickness and ultrasound longissimus dorsi thickness (P≤0.05) respectively. This result
showed that SNPs in MSTN gene could be suggested as good marker for muscling traits in Bali cattle.
Anext generationdelivery systemof bioactive nutrients to dairy cattle for the production and optimization
of new, value added, medicinal, milk products
James Templeman
University of Guelph, Canada
This project takes aim at developing a novel and natural nutrient delivery system that incorporates an active ingredient (holy
basil, HB) into molecular gels. These gels or emulsions are fed to dairy cows alongside their regular diets and are efficiently
transferred to the mammary gland, while resisting degradation during digestion. Gel creation is preformed via emulsification of
a stable wax polymer and a sodium alginate (NaAlg) solution followed by a two-tier gelation process initiated by calcium salts.
The wax complex makes up 25% of the gel and is comprised of rice bran wax (2% w/v) and canola oil. It was selected based on its
stability (<10% degradation) during 48 hours artificial rumen incubation. The NaAlg solution (75% of the gel) is added to the wax
solution to be homogenized and emulsified, creating a low viscosity emulsification. A 9:1 solution (CaCO3:CaCl2) is then added
to our emulsification at the same time as the HB. The calcium salts induce encapsulation of the HB through immediate and long-
term gelation. The insoluble calcium (CaCl2) activates instantaneously, causing rapid gelation, while the insoluble calcium (CaCO3)
activated by a drop in pH is triggered once the gel reaches the cow’s acidic digestive system. The CaCO3 activation creates sustained
gelation; this helps ensure the encapsulated HB survives rumination while on its way to the mammary gland for deposition. This
target-specific delivery system will enhance the functional food properties of milk and can be applied to attain marketable, medicinal
milk products, unique to the industry in their therapeutic qualities.
J Fisheries Livest Prod 2016, 4:2 (Suppl)