20 / 37
Page 74
Notes:
conferenceseries
.com
Volume 9, Issue 9 (Suppl)
J Cancer Sci Ther, an open access journal
ISSN: 1948-5956
World Cancer 2017
October 19-21, 2017
25
th
WORLD CANCER CONFERENCE
October 19-21, 2017 | Rome, Italy
Effect of
WNT7B
in macrophage-stimulated mesothelial cells on ovarian cancer cell adhesion
Seung-Kye Cho
and
Jung-Hye Choi
Kyung Hee University, South Korea
O
varian cancer is a remarkably metastatic disease that is often characterized by widespread peritoneal dissemination.
Recently, several studies have suggested that tumor microenvironment plays a significant role in ovarian cancer
metastasis. However, the interaction between macrophages and mesothelial cells for ovarian cancer metastasis is unclear. We
first investigated the effect of macrophages on gene expression pattern in mesothelial cells. Following treatment of mesothelial
cells with conditioned medium (CM) of macrophages, we conducted a comparative analysis of global expression changes
in mesothelial cells using mRNA sequencing. When compared to that in unstimulated-macrophages, 945 genes were up-
regulated and 777 genes were down-regulated more than 2-fold in macrophage-stimulated mesothelial cells (MSM). Among
the total 1722 genes, 94 genes including
WNT7B
were known to be associated with the regulation of cell adhesion. Interestingly,
knockdown of
WNT7B
using siRNA attenuated the adhesion of ovarian cancer cells to mesothelial cells. These results indicate
that the enhanced levels of
WNT7B
in MSM may play a role in the peritoneal dissemination of ovarian cancer.
Biography
Seung-Kye Cho is pursuing his Master’s degree in Life and Nano-pharmaceutical Sciences at Kyung Hee University. He graduated in Oriental Pharmaceutical
Science from the Kyung Hee University. He has interests in providing evidence supporting the importance of the interplay between ovarian cancer cells and
mesothelial cells, and discovering novel factors of peritoneal dissemination of ovarian cancer.
csk4423@gmail.comSeung-Kye Cho et al., J Cancer Sci Ther 2017, 9:9(Suppl)
DOI: 10.4172/1948-5956-C1-112