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Volume 9, Issue 9 (Suppl) J Cancer Sci Ther, an open access journal
ISSN: 1948-5956 World Cancer 2017
October 19-21, 2017
25th WORLD CANCER CONFERENCE
October 19-21, 2017 | Rome, Italy
Serum uric acid is effectively correlated with the quality of donated red blood cells under blood bank
conditions
Vasileios L Tzounakas1, Dimitrios G Karadimas1, Alkmini T Anastasiadi1, Hara T Georgatzakou1, Eleftheria Kazepidou2, Dimitris Moschovas3, Athanassios
D Velentzas1, Anastasios G. Kriebardis4, Nikolaos E. Zafeiropoulos3, Apostolos Avgeropoulos3, Marilena Lekka2, Konstantinos E Stamoulis5, Issidora S
Papassideri1, and Marianna H Antonelou1
1National & Kapodistrian University of Athens (NKUA), Greece
2Department of Chemistry, University of Ioannina
3Department of Materials Science Engineering, University of Ioannina
4Technological and Educational Institute of Athens
5Hellenic National Blood Center, Acharnes, Greece
Statement of the Problem: During their preservation at blood banks, red blood cells (RBCs) undergo several physiological
alterations/deteriorations collectively known as “RBC storage lesion”. A significant part of the storage lesion is driven by
oxidative stress, while some of its critical aspects are considered donor-related. Having in mind that serum uric acid (UA)
represents almost 60% of the total antioxidant capacity of the donor’s plasma, the aim of this study was to provide evidence
regarding the potential usefulness of UA as a donor-specific marker of storage quality.
Methodology & Theoretical Orientation: For this purpose, 47 non-leukoreduced units of RBC concentrates in CPDA-
1 produced from male regular blood donors were stored for 35 days. Several storage quality parameters (cell shape, redox
homeostasis, extracellular vesicles/EVs etc.) were examined at the beginning (day 2), the middle (day 18) and the end (day 35)
of the storage period. SPSS was used for statistical analysis. Findings: Antioxidant capacity of the blood bags’ supernatant was
correlated with the UA levels i n v i v o (R=0.718, p<10-7) throughout the storage period. A posteriori splitting of the donors in
high- and low-UA groups, revealed statistically lower intracellular ROS and calcium accumulation after the middle of storage
(p<0.05) in the high UA group. Finally, units from high UA donors demonstrated lower levels of irreversibly modified RBCs
(22.5±2.9% vs 27.1±1.6%) and different size distribution of EVs on day 35 of storage (p<0.05).
Conclusion & Significance: Variability in UA levels in vivo is maintained during storage and of note, it seems to be associated
with the redox status and morphology of stored RBCs. Uric acid as a donor’s signature in blood components may be a very
promising candidate biomarker of RBC storage lesion. This study was supported by “IKY FELLOWSHIPS OF EXCELLENCE
FOR POSTGRADUATE STUDIES IN GREECE – SIEMENS PROGRAM” to Vasileios Tzounakas.
Biography
Vasileios L Tzounakas is a Post-doctoral researcher at the Department of Biology (Section of Cell Biology & Biophysics) of the National and Kapodistrian University
of Athens (NKUA). He has obtained Ph.D. in Cell Biology. He has served as reviewer in international journals while his main research interests include blood
transfusion biology (mainly, red blood cell storage lesion in blood products used for transfusion), erythrocyte biology in health and disease and the study of
extracellular vesicles. He has expertise in evaluating the key parameters that affect storage lesion and posttransfusion performance of red blood cells and in the
management of blood supplies in a way that will lead to the individualization of transfusion therapy. In this context, he has focused on the elucidation of storage
lesion’s features that may serve as a donor’s signature, namely “the donor variation effect.
Vasileios L Tzounakas et al., J Cancer Sci Ther 2017, 9:9(Suppl)
DOI: 10.4172/1948-5956-C1-112