4 / 25
Page 25
Notes:
Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
3
rd
World Biotechnology Congress
December 03-04, 2018 Sao Paulo, Brazil
Analysis of the expression of the SlMYB gene during the reproductive development of tomato
(
Solanum lycopersicum
)
Jose Linares, Concha Gomez Mena, Jose Pio Beltran Porter
and
S Chem
Polytechnic University of Valencia, Spain
Statement of the Problem:
In tomato, the development of the fruit is a highly regulated process at the genetic level that
can be negatively affected by changes in environmental conditions, mainly humidity and temperature. The knowledge of the
molecular mechanisms that control the setting and development of the fruit is very important to identify improvement targets
for this crop. Plant development processes are often controlled by networks of regulatory genes that encode transcription
factors. In a previous work of the laboratory, a transcription factor type MYB (SlMYB) was identified whose expression is
induced in androsterile tomato plants whose ovaries develop in the absence of pollination, giving rise to fruits without seeds
(parthenocarpic). By expression analysis, it was determined that this gene is expressed only in developing flowers.
Methodology & Theoretical Orientation:
The
in situ
hybridization technique is very suitable to obtain information about
the expression patterns of genes in plants, also, express promotor of SlMYB with GUS gene in
Arabidopsis
could reveal the
potential of these genes as a biotechnological tool.
Findings:
Involved primary research with the expression pattern of a gene on the floral structure of
Solanum lycopersicum
and
Arabidopsis thaliana
in the laboratory of Dr Concha Gómez-Mena. Our results show that the messenger of this gene is located
in the sporogenic tissue of the anther, in the developing titles and in the transmission tissue of the pistil. On the other hand,
transgenic
Arabidopsis
pSlMYB::GUS lines have been obtained that show a 2kb sequence of the SlMYB gene promoter capable
of directing the expression of the GUS reporter gene to anther and style tissues.
Conclusion & Significance:
One important conclusion of this research is this gene could regulate male and female meiosis.
Biography
Jose Linares Master Degree in Molecular and Cell Plant Biotechnology at the Polytechnic University of Valencia. A biologist at National University of San Marcos
(UNMSM), specialized in genetics and plant biotechnology. Experience in Scientific Research and Intellectual Property (patents and plant varieties); development
of research projects related to the characterization of plant genetic diversity at the cellular, biochemical and genetic level; Knowledge of laboratory techniques
(cytogenetic, biochemical and molecular biology, microbiology, genetic engineering). Working about four years as a Consultant of Intellectual Property related to
plant biology.
jrlinaresgonzales@gmail.comJose Linares et al., J Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C7-106