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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8

World Biotechnology Congress

December 03-04, 2018 Sao Paulo, Brazil

Study of the conditions of encapsulation of piperine in microwave-assisted nanosponges

Jeyson Alan Hermosilla Gajardo

University of La Frontera, Chile

anosponges (NS) is a class of colloidal structures based on hyper-crosslinked polymers that are made up of microscopic

particles with nanometric cavities in which a large variety of substances can be encapsulated. These cavities can

incorporate lipophilic and hydrophilic compounds. The objective of this work was to evaluate the effect of NS with different

degree of crosslinking, the type of solvent (SOLV) used in the loading process and the load ratio PIP-NS (w/w) on the

encapsulation efficiency of piperine (PIP) in the microwave-assisted cyclodextrin‐based nanosponges. The NS was formed

with β-cyclodextrin (β-CD) and diphenyl carbonate (DPC) through the microwave-assisted method. NS of molar ratios 1:2,

1:6 and 1:10 (β-CD-DPC) were used to perform loads of PIP-NS (2:1, 1:1 and 1:2) (w/w). The solvents used in the fillers were

dichloromethane, acetone and ethanol. Characteristic peaks were identified for PIP and displacement of peaks attributable to

inclusion as methylenedioxy phenyl at 928cm-1. ANOVA was performed to determine if there were statistically significant

differences for each of the variables on the encapsulation efficiency response. NS and PIP-NS are significant (p≤0.05), on the

other hand, SOLV was not significant (p≥0.05) on the response. For the NS variable, the molar ratio that presented the best

responses were 1:6=77.12a and 1:10=78.74a followed by 1:2=71.55b. For the PIP-NS variable, the load ratio that presented the

best response was 1:2=84.08a, followed by 2:1=77.28b and finally 1:1=66.05c.

Biography

Jeyson Alan Hermosilla Gajardo is a biotechnologist from the University of La Frontera, Temuco, Chile. He has worked on several projects at the University in

the micro and nanoencapsulation area, currently working on the Fondecyt project N°1160558 “Nanoencapsulation of polyunsaturated fatty acids and pungency

alkaloids using nanosponges as carrier model to deliver lipophilic compounds of high biological value”.

jeyson.hermosilla@ufrontera.cl

Jeyson Alan Hermosilla Gajardo, J Biotechnol Biomater 2018, Volume: 8

DOI: 10.4172/2155-952X-C7-107