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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
3
rd
World Biotechnology Congress
December 03-04, 2018 Sao Paulo, Brazil
Genetic characterization of
Rubus glaucus
Benth progenitors through SNPS and SSR
Ana Maria Lopez Gutierrez
and
Marta Leonor Marulanda Angel
Technological University of Pereira, Colombia
R
osacea family comprise nearly 90 genera, with Rubus genus among them with 750 species (Longhi et al., 2014). This genus
also includes a plant commonly known as
mora de Castilla
or
mora de los Andes
(Rubus glaucus). Genetic variability
of the genus is well documented and has been widely studied considering phenotypic, morphologic, chromosomic and
molecular features, highlighting important aspects such number of chromosomes, polyploidy and hybridization (Alice and
Campbell, 1999).
Rubus glaucus Benth
(
mora de Castilla
) is an agricultural product with one of the biggest potential within
Colombian-Andean Region. In the country, along 2014 it was achieved a total production of 150 thousand tons/year and in
2015, the plant's area accounted 66.770 hectares with average yielding of 11 tons per hectare per year (ENA, 2015). In this
sense, the agricultural-food chain of
mora de Castilla
in Colombia is constituted by associated producers, with the intention of
increasing performances through the behavior evaluation of promising plant material against high impact-phytopathogenic
agents. Accordingly, this work pretends to strengthen the species characterization process of
mora de Castilla
through last
generation molecular markers SSR and SNPs tending to identify tolerance-related genes in
Rubus glaucus
cultivars against
anthracnose caused by
Colletotrichum gloesporioides
through transcriptome analysis (RNAseq) in susceptible species. Hence,
15 SRR markers showed positive amplification with a bi-allelic behavior for 14 of them, giving place to 29 loci and 58 alleles.
Moreover, from 78 SPNs markers, only 36 yielded positive amplification. Obtained sequences showed high homology with
species belonging Rosaceae family, as a result, genetic diversity based on SNPs data consisting in 8837 loci with 1082 effective
alleles and an average polymorphism of 12.49% Revealed that genetic libraries constructed with SNPs and SSR markers showed
high discrimination power for commercial cultivars of
Rubus glaucus
.
Biography
Ana Maria Lopez Gutierrez possesses wide experience into the fields of plant biology and plant genetics. Her work has concentrated in the investigation of plant
diversity using SSR, SNPs and AFLP markers but also tending to the identification of resistance genes in susceptible and tolerant species against bacterial and
fungal infections affecting crops with commercial/food interests.
alopez@utp.edu.coAna Maria Lopez Gutierrez et al., J Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C7-107