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Journal of Biotechnology & Biomaterials | ISSN: 2155-952X | Volume: 8
3rd World Biotechnology Congress
December 03-04, 2018 Sao Paulo, Brazil
Morphologic and genetic characterization of Botrytis cinerea Pers. isolates responsible for gray mold
in Rubus glaucus Benth in Colombia
Liliana Isaza Valencia, Marta Leonor Marulanda Angel and Yeny Paola Zuluaga
Technological University of Pereira, Colombia
Botrytis cinerea Pers. is considered the most common and severe specie within Botrytis genus, a taxon which comprises
301 accepted names (Ferrada et al. 2016; IMA 2016). This fungus induces gray mold or fruit rot in some Rubus species, in
almost all berries crops, but also causes the considerable economic loss in nearly 200 agriculturally important crops (Suresh
et al. 2010; Zhou et al. 2014). Botrytis cinerea is considered as a complex system of species instead of a single variable one
in which have been identified two sympatric groups based onto multiple-gene genealogies (Fournier et al. 2005). All these
factors have created a great intrapopulation genetic variation affecting phenotypical features, such as adaptability, mycelial
growth and pathogenicity. As a result, many studies tried to assess and characterize the genetic diversity of local B. cinerea
species isolated from different cultivars. Thus, this paper pretends to explore and evaluate the diversity of B. cinerea isolates
across Colombian cultivars using morphological and molecular approaches over 50 collected isolates, assessing mycelial
growth (vertical and horizontal elongation), sclerotial distribution, color and SSR genetic diversity. Results showed the high
correlation among both types of growth with statistical differences over the population average for seven strains. Similarly, the
vertical and horizontal size of sclerotium were recorded, finding significant differences inside the studied group. In addition,
genetic characterization driven data resulted in the construction of a phylogenic tree showing a mid-tendency of clustering
depending on the geographical regions of the collection. Finally, fungal isolates of B. cinerea collected in different Colombian
Rubus glaucus Benth cultivars of showed significant differences regarding mycelial growth and genotypic clustering pattern
regarding its origin area. This general behaviour could support further pathogenicity studies in order to understand explore
differential severity among them.
Biography
Liliana Isaza Valencia possesses wide experience into the field of plant tissue culture and in vitro culture of strategic plant species with economic and agricultural
importance, including species of Heliconia and Rubus genus. In addition, she had performed research related to the characterization of genetic diversity of the
mentioned plant species.
Liliana Isaza Valencia et al., J Biotechnol Biomater 2018, Volume: 8
DOI: 10.4172/2155-952X-C7-107