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Volume 7, Issue 6 (Suppl) J Biotechnol Biomater, an open access journal
ISSN: 2155-952X World Biotechnology 2017
December 04-05, 2017
2nd World Biotechnology Congress
December 04-05, 2017 | Sao Paulo, Brazil
Evaluation of flavonoid catechin from an endophytic fungus Curvularia australiensis FC2AP for treating
cervical cancer in female Sprague Dawley rats
Vellingiri Manon Mani1, 2, Arockiam Jeyasundar Parimala Gnana Soundari1, 2 and Kathirvel Preethi
1Hindusthan College of Arts and Science, India
2Bharathiar University, India
Introduction: There is an increasing emergence of bio products from microbes such as metabolites in the fields of medical and
pharmaceuticals. Most of the people in the current world are affected with cancer; especially, women are in major risk of cervical
cancer. In this due, this research has been focused to obtain antioxidants or potential metabolites to treat against the cervical
cancer. Majorly among the microbes, endophytic fungi have been considered as prospective one which produces novel metabolites
substantially. With this in view, the current investigation has been designed for the activity of anti-cancer drug flavonoid catechin in
Sprague Dawley rats.
Materials & Methods: The secondary metabolites have been explored from Curvularia australiensis FC2AP (KR363626) isolated
from Aegle marmelos by statistically optimized fermentation conditions, purified (chromatographic techniques) and characterized
(HRLC- MS/MS, FT-IR and NMR: 1C & 13 C) as a potential secondary metabolite as flavonoid catechin (MM4). The potential product
was taken to assess the acute toxicity in albino mice, anti- inflammatory property in Wistar rats and anti-cancer evaluation in Sprague
Dawley rats by following the CPCSEA standards. All animal procedures were performed in accordance with Institutional Animal
Ethic Committee (IAEC) guidelines, after getting the approval from the Committee for Control and Supervision of Experiment on
Animals (CPCSEA) at KMCH college of Pharmacy, Coimbatore, Tamil Nadu, India.
Results: Through this study, we found that the effective dosage for the survival is 1.25 g/kg (Gp V) and the lethal dosage as 1.5 g/
Kg (Gp IV) in acute toxicity assessment. Above 1.5 g/Kg of the purified compound MM4 produced hyper sensitivity, righting reflex,
tremors and convulsions leading to the death of the animal. The anti-inflammatory analysis resulted with the percentage of inhibition
was found to be 41.09% (300 mg/kg) which was found to be twice than the standard drug indomethacin (20.17%) used whereas,
the Group IV showed only 12.9% inhibition. This proved that the compound flavonoid catechin was efficient against inflammatory
responses. The cervical cancer was induced in female rats using DEN (N-Nitrosodiethylamine) and the treatment was continued with
flavonoid catechin for 180 days. After the stipulated days the rats were taken for hematological, biochemical and histopathological
studies and the results indicated that the compound MM4 has the ability to reduce the tumor by not affecting the nearby non- tumor
cells. Further, the investigation will be carried in higher animals.
Conclusion: This study explores the biomolecule flavonoid catechin from an endophytic fungus to explore the anticancer potentiality
through animal models. This is the first report of flavonoid catechin production from an endophytic fungus C. australiensis has the
anticancer potentiality. This metabolite can be stated as anticancer drug.
Biography
Vellingiri Manon Mani has started up her research on anti-cancer with bioactive microbial metabolites in a passionate aim to achieve the grail for human wellbeing. Her
experience gained on research and teaching has been explored to make the students to get higher knowledge on biotechnological fields. The research gained by her
had a great impact on treating the cervical cancer and moreover the drugs obtained by her research have been implied on the medicinal fields. She has developed a new
technique on the extraction of microbial metabolites and its application as a drug in medicinal fields which is under filed in IPR. The technical approach developed by her
will have highest reach on the research field in future biotechnological field.
Vellingiri Manon Mani et al., J Biotechnol Biomater 2017, 7:6 (Suppl)
DOI: 10.4172/2155-952X-C1-085