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Volume 7, Issue 6 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
World Biotechnology 2017
December 04-05, 2017
2
nd
World Biotechnology Congress
December 04-05, 2017 | Sao Paulo, Brazil
Xylopia aromatic
hexane extract promotes antitumor effects in Ehrlich tumor carcinoma
Izabela N F Gomes
1
, Ana G Silva
2
, Gustavo F F Lima
2
, Maria J F Passos
2
, Tamara R Longatti
2
, Lucas S Fernandes
2
, José A F P Villar
2
, Hélio B Santos
2
, Ralph T
Thomé
2
and
Rosy I M A Ribeiro
2
1
HCB-CPOM, Brazil
2
UFSJ-CCO, Brazil
Introduction:
The
Xylopia aromatica
, a typical Brazilian cerrado species, has been used in several studies due to the biological
properties related to the presence of alkaloids, flavonoids and acetogenins presence in genus
Annonaceae
. In this study, we evaluated
the antitumoral effect of extracts of
X. aromatica
and identify their secondary metabolites.
Methodology: The hexane and ethyl acetate extracts were obtained by liquid-liquid fractionation of hydroalcoholic extract (CE). A
cell line of Ehrlich ascites carcinoma cells (EAC) was obtained from the peritoneal cavity. 2.5x10
5
cells/well and it was distributed and
treated with a serial dilution of 1000
μg.mL -1
to 32.3
μg.mL -1
of extracts. The cells were count at 6, 12, 18 and 24 hours after treatment.
To assay in vivo, the animals were inoculated with 2x10
6
cells/well in suspension (PBS, pH 7.2), in the right flank. The animals were
treated intraperitoneally with 32.3
mg.kg-1
of hexane partition and 32.3
mg.kg-1 of ethyl acetate partition. The tumor growth was
accompanied for 20 days. Secondary metabolites were identified by HPLC-DAD.
Results: All partitions and CE were cytotoxic against EAC
in vitro
. The lowest concentrations of hexane and ethyl acetate partitions
(62.5
mg.mL -1
and 32.3
mg.mL -1
, respectively) were more cytotoxic than other treatments. For the
in vivo
assay, hexane partition
induced a decrease of necrosis area, inflammatory infiltrate and MMP-2 expression. The extracts demonstrated a band characteristic
for phenolic acid (263 nm), flavonoids (255 and 354 nm) and alkaloids (282 and 302 nm).
Conclusion: The study concludes that hexane extract of
X. aromatic
may be a promising natural source for active compounds against
cancer.
Biography
Maria Juliana Ferreira Passos holds a degree in Biochemistry from the Federal University of São João Del-Rei (2013). She has concluded the scientific initiation with the
development of the work entitled "Isolation and characterization of isolated peptides of venom of the snake
Crotalus oreganus abyssus
with pharmacological action on
the control and release of insulin", in two consecutive years as CNPq fellow. She has completed her Master's degree at the Federal University of São João Del Rei by the
Graduate Program in Pharmaceutical Sciences, finalizing the project that involves the research by targets, through the technique of Reverse Vaccinology, that allow the
development of vaccines against
Schistosoma mansoni
. Currently, she is a PhD student in the multicenter program of postgraduate in Biochemistry and Molecular Biology,
with emphasis on the discovery of natural extracts that have antitumor action on human glioma lines.
jusinhamg@yahoo.com.brIzabela N F Gomes et al., J Biotechnol Biomater 2017, 7:6 (Suppl)
DOI: 10.4172/2155-952X-C1-085