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Notes:

Volume 7, Issue 6 (Suppl)

J Biotechnol Biomater, an open access journal

ISSN: 2155-952X

World Biotechnology 2017

December 04-05, 2017

World Biotechnology Congress

December 04-05, 2017 | Sao Paulo, Brazil

Design and testing of duplex-PCR primers for detection of bacterial spot of tomato

Dagmar Stehlikova, Vladislav Curn

and

Pavel Beran

University of South Bohemia in Ceske Budejovice, Czech

his study presents development of duplex-PCR assay for specific detection of plant pathogenic bacteria of

Xanthomonas

genus

causing bacterial spot of tomato and pepper. PCR primers for differentiation of

X. euvesicatoria

and

X. vesicatoria

were developed

based on the DNA sequences of bacterial type strains. Primer pairs were designed and subsequently thoroughly tested and optimized

for parallel detection of the bacteria. Specificity of the primers was tested on a large complex of bacterial strains pathogenic to tomato,

pepper and related crops. Following the described protocol

X. euvesicatoria

and

X. vesicatoria

can be quickly and reliably identified

in a single duplex-PCR assay.

Biography

Dagmar Stehlíková is a Molecular Geneticist with specialization on detection phytopathogenic bacteria. She is most interested in quarantine bacteria

Xanthomonas, Ralsto-

nia, Burkholderia

etc. Techniques that she is expert in molecular methods are loop-mediated isothermal amplification, reatime PCR and multipex PCR.

Dagmar Stehlikova et al., J Biotechnol Biomater 2017, 7:6 (Suppl)

DOI: 10.4172/2155-952X-C1-086