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Volume 7, Issue 6 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
World Biotechnology 2017
December 04-05, 2017
2
nd
World Biotechnology Congress
December 04-05, 2017 | Sao Paulo, Brazil
Cytotype and molecular variability of Myriophyllum L
T Kávová
1
, T Kubátová
1
, Trávníček B
2
and
Prančl J
2
1
University of South Bohemia, Czech
2
Academy of Science of the Czech Republic, Czech
M
yriophyllum L.
(watermilfoil) is the largest genus of the family
Haloragaceae
and belong the most species-rich genera of aquatic
plants. Distinguishing of genus
Myriophyllum
is rather challenging. Despite the apparent taxonomic complexity, almost nothing
is known about the cytogenetic structure of the genus in North America. In our study, we used flow cytometry and chromosome
counting to recognize genome size and DNA ploidy level in species of genus
Myriophyllum
. We analyzed 294 European and 329
North American population samples. All species of genus
Myriophyllum
were found in both areas, except North American
M
.
alterniflorum
. Large cytotype variability (2x, 3x, 4x, 6x, 8x and 9x) was found in the USA and Europe. Cytotype variability was found
in populations of
M
.
heterophyllum
where diploids and triploids were examined. Sympatric growth of diploid and triploid cytotypes
was encountered in one population of
M
.
pinnatum
. Two populations of
M
.
aquaticum
in Europe (Hungary) had cytotype variability
(6x and 8x) whereas all N. American populations were octoploids. Cytotype variability was also found in populations of
M
.
sibiricum
where hexaploids and nonaploids were observed. There was clear geografic isolation showing
M
.
sibiricum
as a hexaploids only in
N. American and nonaploids in European populations. Only one nonaploid specimen of
M
.
sibiricum
was found in N. American
populations and just one hexaploid of
M
.
sibiricum
in the European population. Nonaploids in USA and hexaploids in Europe of M.
sibiricum
were detected for the first time. Genome size (2C) ranged from 0.41 pg in diploid
M
.
humile
(2n=14) to 2.66 pg in nonaploid
M
.
sibiricum
(2n=63). These findings give evidence that a detailed study of cytotype composition. Last but not least, studies of ploidy
variation have repeatedly proved necessary to elucidate the mechanisms of triggering the invasive behavior in plants.
Biography
T Kávová is a Molecular Geneticist with specialization on population-genetic studies. She is most interested in ecological topic with the implantation of new techniques in
molecular analyses. Techniques that she perfectly controls range from basic lab work, through complete knowledge of molecular genetic techniques including sequencing
to current flow cytometry specialization. The topic of her thesis is genetic and cytogenetic variability of
Myriophyllum L
. in the native and invasive area of the genus..
tereza.kavova@seznam.czT Kávová et al., J Biotechnol Biomater 2017, 7:6 (Suppl)
DOI: 10.4172/2155-952X-C1-086