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Volume10, Issue 12 (Suppl)
J Proteomics Bioinform, an open access journal
ISSN: 0974-276X
Page 114
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World Biomarkers & Pharma Biotech 2017
December 07-09, 2017
December 07-09, 2017 | Madrid, Spain
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20
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International Conference on
PHARMACEUTICAL BIOTECHNOLOGY
9
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WORLD BIOMARKERS CONGRESS
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J Proteomics Bioinform 2017, 10:12(Suppl)
DOI: 10.4172/0974-276X-C1-110
Erythrocyte membrane disorganization: Potent and cost-effective biomarker in early diagnosis of cervical
cancer
Sagarika Mukhopadhyay
University of Kolkata, India
C
ervical cancer is the fifth most common cancer of the world, and poses a major public health problem. This paper explores
a de novo observation that cervical cancer induced oxidative stress is responsible for erythrocyte membrane disorganization
in the patients of advanced stage of clinical progression of the disease. The study of carbonyl content, antioxidant enzymes, lipid
peroxidation, membrane fluidity and SDS-PAGE of erythrocyte membrane protein has been conducted on 94 adult cervical cancer
patients and an equal number of age and sex matched normal subjects. Lipid peroxidation of erythrocyte membrane is observed to be
enhanced and antioxidant enzyme activity alters significantly in the pathologic samples. Increased membrane fluidity is indicated by
analysis of fluorescence depolarization using 1,6 diphenyl-1,3,5 hexatriene compared to healthy controls. The transition temperature
of membrane lipids from gel to sol phase transition is observed to be shifted from 35°C (control subjects) to 25°C (cervical cancer
patients). Degradation of the spectrin band is evidenced in SDS-PAGE of the membrane protein profile of the diseased subjects. It can
be elucidated that cervical cancer induces oxidative stress in erythrocytes which finally results in increased erythrocyte membrane
fluidity, altered phase transition temperature and modified protein profile. This is an original work on the importance of the protein
profile of RBC membrane. These findings can be used as a characteristic signature of the red blood cell membranes and may be used
for the diagnosis of cervical cancer.
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