veterinary-summit-2016_posters-accepted-abstracts

15 / 17

Page 59
Veterinary Summit 2016
November 14-16, 2016
Volume 7 Issue 7(Suppl) J Vet Sci Technol
ISSN: 2157-7579 JVST, an open access journal
conferenceseries .com
November 14-16, 2016 Atlanta, USA
6th Global Veterinary Summit
J Vet Sci Technol 2016, 7:7(Suppl)
Effects of chromium (III) picolinate and chromium (III) picolinate nanoparticles supplementation
on growth performance, organs weight and immune function in cyclic heat stressed broiler chickens
Omid Hamidi1, Mohammad Chamani1, Hasan Ghahri1, Ali Asghar Sadeghi1 and Hassan Malekinejad2
1Islamic Azad University, Iran
2Urmia University, Iran
The experiment is conducted to investigate the effects of dietary chromium (III) picolinate (CrPic) and chromium (III)
picolinate nanoparticles (NanoCrPic) supplementation on growth performance, organs weight and immune function of
broilers exposed to heat stress. Heat stress (36 °C) was applied for 10 hours per day from the 21st to the 42nd days. Among 8
experimental treatments; only group T1 represented the non-heat stressed control group fed with a basal diet in comfort zone
whereas group T2 represented the heat stressed control group fed with a basal diet. Heat stressed T3, T4, T5 groups were fed with
basal diet supplemented with 500, 1.000, 1.500 ppb of CrPic/kg while T6, T7, T8 groups were fed with basal diet supplemented
with 500, 1.000, 1.500 ppb of NanoCrPic/kg respectively. Results of the current experiment showed that the non-heat stressed
group had a higher final BW, daily weight gain and daily feed intake compared with heat stressed groups during the experiment
period (d 21-42), among heat stressed groups, FCR values improved by supplementation of Cr into the diet. NanoCrPic 1.500
treatment had the lowest (P<0.05) FCR (2.14) of the total experimental period among heat stressed groups. The liver weight
values of the day 35 of experiment differed significantly (P<0.05). Serum complement component C3 of experimental broilers was
severely affected by the Cr supplementation. The results indicated that the nanoparticle supplementation might be an influential
method for reduction of heat stress induced disorders which may attribute to the lowering of FCR and provoking the hepatic
related alteration including the liver weight.
Antibiotic susceptibility and molecular identification of antibiotic resistance genes of staphylococci
isolated from bovine mastitis in central region of Algeria
Saidi Radhwane1, Cantekin Zafer2, Khelef Djamel 3, Ergun Yasar2, Kaidi Rachid1 and Solmaz Hasan4
1University Saad Dahleb, Algeria
2Mustafa Kemal University, Turkey
3Higher National Veterinary School of Algiers, Algeria
4Yuzuncu Yil University, Turkey
The study was carried out to investigate the phenotypic and genotypic identification of in vitro antimicrobial susceptibility of
21 Staphylococci (10 Staphylococcus aureus and 11 Coagulase Negative Staphylococci) isolated from bovine mastitis to 12
antimicrobial drugs frequently using in veterinary medicine in Algeria. Isolates of staphylococci from bovine mastitis were tested
for antibiotics with disc-diffusion method according to the National Committee for Clinical Laboratory Standards guidelines in
theMueller-Hinton agar and resistant genes mecA, blaZ, aac-aph, ermA, ermC, tetK and tetM were detected by PCR. Staphylococci
isolates showed high resistance to penicillin (95.23%), oxacillin (80.95%), clindamycine (80.95%) and erythromycin (76.19%) but,
no resistance of all these strains was detected for gentamicin. Among 21 isolates of Staphylococci, 20 were found to be methicillin
and multidrug resistant. Multidrug resistant strains exhibited several antibiogram patterns (antibiotic I to XIII). The distribution
of antibiotic-resistant genes was mecA (100%), tetM (100) followed by blaZ (42.85%). In the present work, the significant
determination was the high prevalence of methicillin-resistant Staphylococci, which were resistant to multiple antibiotics. The
finding of methicillin-resistant staphylococci (MRS) from bovine mastitis is the first report in Algeria and revealed the status of
resistant isolates in herd that might be helpful in treatment, controlling of resistant strains and for deciding culling of cows.