veterinary-summit-2016_posters-accepted-abstracts

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Veterinary Summit 2016

November 14-16, 2016

Volume 7 Issue 7(Suppl)

J Vet Sci Technol

ISSN: 2157-7579 JVST, an open access journal

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November 14-16, 2016 Atlanta, USA

Global Veterinary Summit

J Vet Sci Technol 2016, 7:7(Suppl)

Effect of kisspeptin-10 on serum testosterone levels in stallion, donkey and mule

Irfan Zia Qureshi and Rana Waseem Akhtar

Quaid-i-Azam University, Pakistan

his study was conducted to determine the response of serum testosterone in male equines following bolus doses of Kisspeptin,

hCG and LH. Male animals of each equine species (stallions, donkeys and mules) were divided into four groups. Kisspeptin

-10 was administered intravenously into the jugular vein of all animals. Group-1: Treated with 3 ml (0.95% saline); Group-2:

Treated with 50 µg Kisspeptin; Group-3: Treated with 2500 IU hCG and, Group-4 treated with 400 µg LH. Serum testosterone

levels among different treatment groups were compared through one way ANOVA, P<0.05 was taken as significant difference.

Administration of Kisspeptin to all the three species i.e., stallions, donkeys and mules led to significant (P<0.001) increase in

testosterone concentration at 240 min post dose as compared to the saline treated group. Upon LH administration a highly

significant increase (P<0.001) in serum testosterone concentrations was noticeable at 240 min in stallions, donkeys and mules as

compared to pre dose testosterone concentrations. In case of hCG treatment, the concentration of serum testosterone was also

found significantly greater in stallions (P<0.05), in donkeys (P<0.01) and in mules (P<0.001) at 240 min post dose as compared

to the pre-dose concentration. Administration of Kisspeptin and other reproduction related hormones to male equines causes

significant increase in serum testosterone concentration demonstrating similar effect of all the peptides.

Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against

oxidative damage by improving antioxidant response and mitochondrial function promoting

wound healing

Jose M Alvarez-Suarez

Universidad de Las Americas, Ecuador

xcessive amounts of free radicals are deleterious for cells, resulting in cell damage, affecting the wound healing process and

causing premature ageing or even neoplastic transformation. Here the capacity of Manuka honey (MH) to protect against

oxidative damage and improve the process of skin wound healing was investigated. Up to 16 compounds were identified in MH

with leptosin derivatives and methyl syringate as the major ones. MH protected against apoptosis, intracellular ROS production

and lipid and protein oxidative damage. MH also protected mitochondrial functionality, promoted cell proliferation and activated

the AMPK/Nrf2/ARE signaling pathway, as well as the expression of the antioxidant enzymes such as SOD and CAT. Here we

describe for the first time that one of the possible mechanisms by which MH exhibits its ability to promote wound healing could

be due to its capacity to improve the antioxidant response by activating AMPK phosphorylation and the ARE response.