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Volume 5, Issue 2 (Suppl) J Tradi Med Clin Natur
ISSN: JTMCN, an open access journal
Page 32
Notes:
Traditional Medicine 2016
September 14-16, 2016
conferenceseries.com
September 14-16, 2016 Amsterdam, Netherlands
6th International Conference and Exhibition on
Traditional & Alternative Medicine
Comparative in vitro cytotoxic, anti-inflammatory and anti-microbiological activities of two indigenous
Venda medicinal plants
Muendi T Sigidi, C P Anokwuru, T Zininga, MP Tshisikawe, A Shonhai, IDI Ramaite, AN Traoré and N Potgieter
University of Venda, South Africa
The Vhembe region of the Limpopo province has a rich tradition of medicinal plants use. Traditionally, boiled roots of Ziziphus
mucronata are used in the treatment of boils, general swelling and other skin infections. A combination of leaf paste and root
infusion treats measles, dysentery, chest complains, and gland swelling. Pterocarpus angolensis is famous for the treatment of
menorrhagia, infertility in women, wounds and pain management. The purpose of the present study was to compare the cytotoxicity,
anti-inflammatory potential and anti-microbial activities of Ziziphus mucronata and Pterocarpus angolensis from the Vhembe region.
U937, MeWo, Vero and RAW 264.7 cells were treated to various concentrations (50, 100, or 125 or 250 μg/ml depending on assays)
of Ziziphus mucronata and Pterocarpus angolensis. Cytotoxicity assay was done using MTT; Anti-inflammatory activity was assessed
using NO production; Anti-bacterial activity was done using the Micro-Broth dilution method and Anti-mycobacteria activity was
determined using the Alamar Blue Method while RT activity was measured by ELISA. Cytotoxicity results showed that Pterocarpus
was more toxic than Ziziphus as observed in the Vero and MeWo cells; however both displayed toxicity towards a Human cancer cell
line. Both extracts did not inhibit nitrate production but induced significant increase in macrophage activation. The plant extracts
have shown anti-tuberculosis activity at concentrations >500µg/ml and there was moderation inhibition of HIV replication. The
results obtained indicated that the extracts have pro-inflammatory properties, and the observed toxicity on malignant cell lines must
be investigated further for promising anti-cancer drug therapy.
Biography
Muendi T Sigidi is a PhD student from the University of Venda in South Africa. She worked for the National Institute of Communicable Diseases (NICD), Mycology
Reference Unit for 3 years as a Scientist. Currently, she is a part time Lecture in the Department of Microbiology in the University of Venda. She has commenced
the PhD degree in January 2014 and its due for completion is at the end of 2016.
Muendi T Sigidi et al., J Tradi Med Clin Natur 2016, 5:2 (Suppl)