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Volume 5, Issue 2 (Suppl) J Tradi Med Clin Natur
ISSN: JTMCN, an open access journal
Page 36
Notes:
Traditional Medicine 2016
September 14-16, 2016
conferenceseries.com
September 14-16, 2016 Amsterdam, Netherlands
6th International Conference and Exhibition on
Traditional & Alternative Medicine
Decreased interleukin-4 (IL-4), IL-10 and IgE level of type I hypersensitive mice using scopoloetin isolated
from noni fruit (Morinda citrifolia L)
Yufri Aldi, Elliza Nasrul, Yanwirasti, Dian Handayani and Dachriyanus
Fakultas Farmasi, Indonesia
An in vivo study of the activity of scopoletin isolated from noni fruit (Morinda citrifolia L.) on the level of interleukin-4 (IL-4),
IL-10 and IgE in type I hypersensitive male Swiss-Webster mice has been carried out. Scopoletin was isolated from dried noni
powder by soxhletation method using dichloromethane, separated by column chromatography using silica gel as stationary phase
and n-hexane-ethyl acetate (1:4) as mobile phase, then purified by column chromatography using Sephadex LH20 as stationary
phase and methanol as mobile phase. Type I hypersensitive male mice were obtained by ovalbumin sensitization. Animal model were
divided into 5 groups: negative control group, positive control group, and scopoletin-treated group (1; 3; and 10 mg/kg). The results
showed that scopoletin at doses of 1, 3 and 10 mg/kg decreased the level of IL-4 of type I hypersensitive mice significantly (p<0.01).
The scopoletin at the dose of 10 mg/kg decreased the serum level of IL-4, IL-10, and IgE (P<0.01) to the normal level. The ability of
scopoletin to decrease IL-4 and IgE concentration of type I hypersensitive mice to its normal state was shown by dose of 10 mg/kg BW
(p>0.05), while for IL-10 concentration, the decrease until its normal level was shown by dose of 3 mg/kg BW (p<0.05).
Biography
Yufri Aldi is a Lecturer at the Faculty of Pharmacy, University of Andalas. He completed his PhD in 2013 at Andalas University. His research is in the field of
Farmaco-Immunology.
Yufri Aldi et al., J Tradi Med Clin Natur 2016, 5:2 (Suppl)