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Volume 4 Toxicology: Open Access
ISSN: 2476-2067 Toxicology Congress 2018
March 12-14, 2018
March 12-14, 2018 Singapore
14th World Congress on
Toxicology and Pharmacology
The evaluation of hypoglycemic effects of some plants extract on induced diabetes in mice
Alexandru Octavian Doma, Romeo T Cristina, Eugenia Dumitrescu, Olga Rada and F Muselin
Banat’s University of Agricultural Sciences and Veterinary Medicine, King Michael I of Romania (BUASVM) Timisoara, Romania
Statement of the Problem: Diabetes mellitus is a chronic disease that
is characterized by a relative or absolute lack of insulin, resulting in
hyperglycemia, being one of the most frequent diseases. The purpose of this
study was to determine the hypoglycemic effect of some plats extract on
Streptozotocin induced diabetes in mice.
Methodology: The study was conducted on 35 BALB/c mice divided in five
groups: C- control group receiving distillated water, DC - diabetic control
receiving distillated water, E1 – diabetic mice receiving 10% Arctium lappa
extract, E2 - diabetic mice receiving 1% Betula pendula lyophilizate extract,
E3 - diabetic mice receiving 5% Althaea officinalis extract. The diabetes was
induced by I.P. administration of 200 mg/kg bw Streptozotocin is single
dose, mice with glycaemia over 150 mg/dl were considered diabetic and those with over 200 mg/dl were considered to have
severe diabetes.
Findings: In diabetic rats we observed a significantly (p<0.05) increase of body weight comparative to control and also we
observed a significant (p<0.05) increase of water consumption in diabetic rats in the first 24 hours, followed by a decrease
of these possible due to the change in taste especially in E2 group. Administration of plants extract in groups E1, E2 and
E3, decreased significantly (p<0.05) the glycaemia comparative to DC group reaching the values to C group. The decrease
of glycaemia in groups that received plants extract was graduated started after 24 hours after exposure until 120 hours after
exposure: E1/DC 72 hours: -27.52%; E1/DC 120 hours: -41.02%; E2/DC 72 hours: -32.58%; 120 hours: -43.07%; E3/DC
72 hours: -34.83%; E3/DC 120 hours: -37.43%.
Conclusion: Administration of studied plants extract proven to have a good hypoglycemic effect and could be recommended
for the control of glycaemia.
References
1. Ahangarpour A, Heidari H, Oroojan AA, Mirzavandi F, Esfehani KN, Mohammadi ZD (2017) Antidiabetic, hypolipidemic
and hepatoprotective effects of Arctium lappa root’s hydro-alcoholic extract on nicotinamide-streptozotocin induced type
2 model of diabetes in male mice. Avicenna J Phytomed ; 7(2): 169–179.
2. Glastras SJ, Chen H, Teh R, McGrath RT, Chen J, Pollock CA, Wong MG, Saad S (2016) Mouse Models of Diabetes,
Obesity and Related Kidney Disease. PlosOne ; 1-15, DOI:10.1371/journal.pone.0162131.
3. King JFA (2012) The use of animal models in diabetes research, Review. British Journal of Pharmacology; 166: 877–894.
4. Kooti W, Farokhipour M, Asadzadeh Z, Ashtary-Larky D, Asadi-Samani M (2016) The role of medicinal plants in the
treatment of diabetes: a systematic review. Electron Physician ; 8(1): 1832–1842.
5. Park CJ, Han JS (2015) Hypoglycemic effect of jicama (Pachyrhizus erosus) extract on streptozotocin-induced diabetic
mice. Prev Nutr Food Sci.; 20(2): 88–93.
Biography
Alexandru Octavian Doma is pursuing his PhD in Faculty of Veterinary Medicine from Banat’s University of Agricultural Sciences and Veterinary Medicine, King
Michael I of Romania from Timisoara. He has built his experience in research, evaluation, teaching during the PhD period. He is the Secretary of Romanian Society
for Trace Elements in Medicine.
Alexandru Octavian Doma et al., Toxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-006
Figure-1: Schematic exposure of different plants on diabetic
mice glycaemia