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Volume 4 Toxicology: Open Access
ISSN: 2476-2067 Toxicology Congress 2018
March 12-14, 2018
March 12-14, 2018 Singapore
14th World Congress on
Toxicology and Pharmacology
The antioxidant effect of resveratrol in cisplatin induced oxidative stress
Florin Muselin1, Eugenia Dumitrescu1, Zeno Garban2 Alexandru O Doma1 , Alexandra Trif11 and Romeo T Cristina1
1Banat University of Agricultural Sciences and Veterinary Medicine, Romania
2Romanian Academy, Romania
Statement of the Problem: Cisplatin is one of the most used cytostatic with
a broad antitumor spectrum. The uses of different cytostatic are followed by
release of reactive oxygen species (ROS) responsible for the adverse effect
of the chemotherapy. The purpose of this study was to determine if the
resveratrol administration can reduce the cisplatin induced oxidative stress.
Methodology: The study was conducted on 24 Wistar rats divided in four
groups: C - the control group receiving 1 ml of physiological saline I.P.,
CR - control blank group receiving 20 mg/kg resveratrol I.P., CP - receiving
cisplatin 10mg/kg I.P. and CP+R receiving combination of 10mg/kg cisplatin
and 20 mg/kg resveratrol I.P. At the end of experiment were analyzed the
biomarkers of oxidative stress enzymes: Glutathione (GSH), glutathione reductase (GSH-r), glutathione peroxidase (GSH-px),
catalase (CAT), superoxide dismutase (SOD) and malondialdehyde (MDA).
Findings: Administration of cisplatin was followed by significant decrease of GSH (-29.44%, p<0.01), GSH-r (-31.88%,
p<0.0001), CAT (-52.28%, p<0.0001) and increase of GSH-Px (+31.25%, p<0.001), SOD (+11.27%, p>0.05) and (+24.05%,
p<0.05) comparative to control group. In case of cisplatin combined with resveratrol administration, the majority of biomarker
enzymes of oxidative stress presented not significant (p>0.05) differences comparative to control group (GSH: -4.04%, GSH-r:
-1.96%, GSH-px: +1.97%, SOD: -1.92%, MDA: +10.31%), exception in case of CAT which remains significantly lower than
control (-21.62%, p<0.01).
Conclusion: Analyzing the dynamic of enzymes biomarkers of oxidative stress we can say that administration of resveratrol
can reduce the ROS formation and has a good effect as antioxidant in case of cisplatin administration.
References
1. Kursvietiene L, Staneviciene I, Mongirdiene A, Bernatoniene J (2016) Multiplicity of effects and health benefits of
resveratrol. Medicina; 52(3): 148-155.
2. Kuhad A, Pilkhwal S, Sharma S, Tirkey N, Chopra K (2007) Effect of curcumin on inflammation and oxidative stress in
cisplatin-induced experimental nephrotoxicity. J Agric Food Chem; 55(25): 10150-5.
3. Rjiba-Touati K, Boussema IA, Belarbia A, Achour A, BachaH (2011) Protective effect of recombinant human erythropoietin
against cisplatin-induced oxidative stress and nephrotoxicity in rat kidney. Int J Toxicol; 30(5): 510-517.
4. Tuan BT, Visacri MB, Amaral LS, Baldini D, Ferrari GB, Quintanilha JCF, Pincinato EC, Mazzola PG, Lima CSP, Moriel P
(2016) Effects of high-dose Cisplatin chemotherapy and conventional radiotherapy on urinary oxidative and nitrosative
stress biomarkers in patients with head and neck cancer. Basic and Clin Toxicol and Pharmacol; 118(1): 83-86.
5. Valdecantos MP, Pérez-Matute P, Quintero P, Martínez JA (2010) Vitamin C, resveratrol and lipoic acid actions on isolated
rat liver mitochondria: all antioxidants but different. Redox Rep; 15(5): 207-216.
Biography
Florin Muselin has his expertise in heavy metals reproductive toxicology, oxidative stress and medicinal and poisonous plants. He is an Associate Professor at
Faculty of Veterinary Medicine from BUASMV Timisoaara, Romania.
Florin Muselin et al., Toxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-006
Figure-1: Schematic reaction of the cisplatin administration,
production of reactive oxygen species and resveratrol.