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conferenceseries
.com
Volume 4
Toxicology: Open Access
ISSN: 2476-2067
Toxicology Congress 2018
March 12-14, 2018
March 12-14, 2018 Singapore
14
th
World Congress on
Toxicology and Pharmacology
Anti-photoaging effect of Crocin: Its molecular mechanism in UVB-irradiated keratinocytes and
human dermal fibroblasts
Byung-Mu Lee, Min Kyung Shin and Hyo Sun Suh
Sungkyunkwan University, South Korea
S
olar ultraviolet (UV) radiation, particularly UVB radiation, is the major cause of photoaging, the most damaging effect of
which is skin carcinogenesis. UVB-induced oxidative stress plays a crucial role in initiating and promoting cell signaling
involved in aging. Specifically, photoaging results from the up-regulation of metalloproteinases (MMPs) via the activation
of activator protein-1 (AP-1) and subsequent collagen breakdown in the skin. This study demonstrates the protective effect
of Crocin, an active carotenoid component of
Crocus sativus
L and
Gardenia jasminoides
E, on UVB-induced photoaging.
Using HaCaT human keratinocytes and human dermal fibroblasts (HDFs), we evaluated Crocin’s anti-photoaging effect by
conducting 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, western blots, enzyme assays and
fluorescence-activated cell sorting. In keratinocytes, crocin significantly inhibited UVB-induced oxidative stress and apoptosis
by suppressing reactive oxygen species (ROS) generation. Furthermore, it significantly increased the activity of antioxidant
enzymes regulated by the transcription factor, Nrf2. In fibroblasts, Crocin significantly attenuated UVB-enhanced MMP
expression by inhibiting AP-1 activity in a dose-dependent manner. In addition, it significantly promoted the synthesis of
collagen and elastin in HDF cells. Taken together, these results suggest that Crocin prevents UVB-induced photoaging in
keratinocytes and fibroblasts by suppressing ROS generation and regulating gene expression.
bmlee@skku.eduToxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-006