28 / 36
Page 96
Notes:
conferenceseries
.com
Volume 4
Toxicology: Open Access
ISSN: 2476-2067
Toxicology Congress 2018
March 12-14, 2018
March 12-14, 2018 Singapore
14
th
World Congress on
Toxicology and Pharmacology
Development of solid phase extraction and HPLC method for simultaneous estimation Ilaprazole and
Glimepiride in rat plasma: Application to pharmacokinetic studies
A P Dewani
1
, A S Tripathi
1
, P G Shelke
1
, R L Bakal
2
and A V Chandewar
1
1
Pataldhamal Wadhwani College of Pharmacy, India
2
KYDSCT’S College of Pharmacy, India
A
novel, simple and MS compatible high-performance liquid chromatography (HPLC) method is reported for the
simultaneous estimation of ilaprazole (ILA) and glimepiride (GLM) in rat plasma. The bio-analytical procedure
involves extraction of ILA, GLM and internal standard (IS) from rat plasma with a solid phase extraction (SPE) process. The
chromatographic analysis was performed on Waters-600 system using a isocratic mobile phase comprising methanol:water
(80:20 % v/v) with pH of water modified to 3 using formic acid at a flow rate of 1.0mL/min and Kinetex C
18
column maintained
at 30±1 °C. The signals were monitored using a PDA detector set at 225nm. IS, ilaprazole and glimepiride eluted at 2.04, 4.7
and 7.4min respectively and the total run time was 10min. Method validation was performed as per US Food and Drug
Administration guidelines and the results met the acceptance criteria. The calibration curve was linear over a concentration
range of 10-600ng/mL (r
2
=0.999). The intra- and inter-day precisions for ILA and GLM were (%RSD values) in the range
of 1.52-9.74 and 1.52-11.76%, respectively, in rat plasma. The method was successfully applied in pharmacokinetic studies
followed by oral administration of GLM and ILA in rats.
anildewani007@gmail.comToxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-006