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Volume 3, Issue 1 (Suppl) Toxicol Open Access
ISSN: 2476-2067 TYOA, an open access journal Toxicology Congress 2017
April 13-15, 2017
April 13-15, 2017 Dubai, UAE
8th World Congress on
Toxicology and Pharmacology
Evaluation of in vitro degradation rate of hyaluronic acid-based hydrogel cross-linked with 1,
4-butanediol diglycidyl ether (BDDE) using RP-HPLC and UV-Vis spectroscopy
Mohammed Al Sibani1, Ahmed Al Harassi2 and Reinhard H H Neubert1
1Martin Luther University Halle-Wittenberg, Germany
2University of Nizwa, Oman
Ahyaluronic acid (HA) was cross-linked with 1, 4-butanediol diglycidyl ether to produce nine BDDE-HA hydrogels. The
degradation rates of six hydrogels were evaluated by HPLC and UV-Visible spectroscopy. The percentage amount of
N-acetyl glucosamine (NAG) obtained after one-day enzymatic digestion to the total amount obtained after complete digestion
was an indicative of the degradation rate of each hydrogel. The results were calculated with 95% confidence interval and
showed (62.6%±12.3 w/w), precision value % R.S.D=7.95, average recovery=81.0%, LOD=6.4 µg/ml for HPLC and (63.3±13.9
w/w), precision value %R.S.D=8.83, average recovery=83.1%, LOD=5.4 µg/ml for UV method. The two methods showed also
good linearity with correlation coefficients (R2) of 0.998 and 0.9995 for HPLC and UV method, respectively. For a comparison
purpose, the other three hydrogels were rated using the conventional weight loss method which showed relatively higher
degradation rates with an average of (73.4%±5.7 w/w), %R.S.D=3.13. Statistical analysis revealed that there was no significant
difference between HPLC and UV-Visible methods, however, these values differed significantly (p<0.05) from the value
obtained from the weight loss method.
Biography
Mohammed Al Sibani has completed his Master’s degree in 2008 from Huddersfield University, UK in the field of Analytical Chemistry. Currently, he is a PhD
candidate since July 2013 at the Department of Pharmacy, University of Halle-Wittenberg, Germany and his thesis topic is related to the enhancement of stability
and viscoelastic properties of HA dermal fillers cross-linked with BDDE. He has worked as an Analysis and Application Technician in mass spectrometry lab at
Nizwa University for the last 6 years.
Mohammed Al Sibani et al., Toxicol Open Access 2017, 3:1 (Suppl)