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Volume 8 Journal of Clinical & Experimental Pharmacology Pharmacology and Medicinal Chemistry 2018
October 18-19, 2018
October 18-19, 2018 Dubai, UAE
International Conference on
18th International Conference on
&
Joint Meeting on
Pharmacology and Toxicology
Medicinal and Pharmaceutical Chemistry
Nigella sativa and thymoquinone ameliorate memory impairment and neuro-inflammation in Aβ-
induced rat model of Alzheimer's disease
Saliha Aysenur Cam1, Fatma Uysal1, Hayriye Tatli Dogan1, Ali Parlar 2, Ayhan Cetinkaya3 and Seyfullah Oktay Arslan1
1Ankara Yildirim Beyazit University, Turkey
2Adiyaman University, Turkey
3Bolu Abant Izzet Baysal University, Turkey
Alzheimer’s disease (AD) currently is one of the major healthcare issues worldwide. Unfortunately current therapies for
Alzheimer’s disease do not modify the course of disease. Nigella sativa and its active constituent Thymoquinone (TQ) may
have anti-neuro=-inflammatory actions. The aim of the present study was to investigate possible protective effects of Nigella
sativa oil (NSO) and TQ on Aβ42 -induced AD models of rats. Intrahippocampal injection of Aβ42 peptide provides glial cell
responses and causes neuro-inflammation. NSO and TQ were orally administered daily for 7 days before and for 10 days
after bilateral intrahippocampal Aβ injection. To investigate whether NSO or TQ improve cognition, Passive Avoidance (PA)
and Morris Water Maze (MWM) behavioral tests were performed 10 days later Aβ injection to asses learning and memory
of rats. After the probe test the brain tissues were collected. Immunohistochemical staining with Iba1, GFAP and Caspase-3
antibody and ELISA analysis of TNF-α and IL-1β levels on hippocampal tissue were performed. The oral treatment with
NSO or TQ significantly reduced cognitive impairments in behavioral tests both MWM and PA. Immuno-staining results
revealed that both NSO and TQ reduced microglial and astrocytic activation increased with Aβ injection. Measurements of
pro-inflammatory cytokines in hippocampal tissue of Aβ-injected rats showed an elevation of TNF-α and IL-1β levels. These
changes were significantly reversed by NSO and TQ treatment. In conclusion results represent that NSO and TQ can improve
Aβ-induced cognitive impairments by inhibiting neuro-inflammation. NSO and TQ recommended as a candidate for further
investigation in treatment of AD.
Biography
Saliha Aysenur Cam has completed his Graduation from Hacettepe University, Faculty of Pharmacy. He is pursuing his PhD in Pharmacology Department at Ankara
Yildirim Beyazit University. He currently works as a Lecturer at Ankara Yildirim Beyazit University. His primary area of interest is neuropharmacology.
Saliha Aysenur Cam , Clin Exp Pharmacol 2018, Volume 8
DOI: 10.4172/2161-1459-C3-034