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Volume 8
Journal of Clinical & Experimental Pharmacology
Pharmacology and Medicinal Chemistry 2018
October 18-19, 2018
October 18-19, 2018 Dubai, UAE
International Conference on
18
th
International Conference on
&
Joint Meeting on
Pharmacology and Toxicology
Medicinal and Pharmaceutical Chemistry
Development of spectrophotometric methods for the analysis of nicotinamide in bulk and dosage forms
Noon Abubakr Abdelrahman Kamil, Shaza W. Shantier, Elrasheed A. Gadkariem
Fatima College of Health Sciences, UAE
T
his study was aimed to develop simple, sensitive and accurate zero (0D), first (1D)
and second (2D) order derivative spectrophotometric methods for the analysis
of nicotinamide in bulk and dosage forms. Methods: The zero-order spectrum of
nicotinamide aqueous solution was measured at 262 nm against its blank. This spectrum
was differentiated instrumentally to generate the first and second derivative spectra
which were measured at 272 nm and 278 nm, respectively. The developed methods were
validated as per ICH guidelines.The absorbance ratio of nicotinamide absorbance values
at 214 nm and 262 nm was also determined. Regression data of the developed methods
obeyed Beer’s law over the concentration range 10-50 μg/ml with a good correlation
coefficient (not less than 0.998). The developed methods demonstrated good inter-day
and intra-day precision at the three modes. The obtained recovery percentage (99.2 ±
2.6%, n=3) reflected freedom from interference by the excipients. The absorbance ratio
for nicotinamide at 214 nm and 262 nm was found to be in the range between 2.8- 3.2
which can be used as identification test for Nicotinamide (qualitative analysis).
Conclusion:
The statistical validation at 95% confidence level proved the sensitivity,
accuracy and precision of the developed methods
Recent Publications (minimum 5)
1. Noon K, Shaza S, ElrasheedG (2018) Development of SpectrophotometricMethods
for the Analysis of Nicotinamide in Bulk and Dosage Forms. International journal
of pharmaceutical research and bio-sciences Volume 7(3): 1-10.
2. Elrasheed G, Noon K, Al Obeid H (2009) A new spectrophotometric method for
the determination of methyldopa. Saudi Pharmaceutical Journal 17, 289– 293
Biography
Noon Abubakr Abdelrahman Kamil has her expertise in pharmaceutical chemistry drug analysis and passion in research and pharmacy education. Her research on
drug analysis creates new methods of drug analysis using simple and accurate ways. She has more than ten years of teaching pharmacy students.
noonkamil@gmail.comNoon Abubakr Abdelrahman Kamil et al., Clin Exp Pharmacol 2018, Volume 8
DOI: 10.4172/2161-1459-C3-034
Figure 1-3:
2
D,
1
D, 0D spectrum of
NIC solution (20 µg/ml)