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Volume 7, Issue 4 (Suppl)
Clin Exp Pharmacol
ISSN: 2161-1459 CPECR, an open access journal
Pharmacology Congress 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
8
th
World Congress on
Pharmacology and Toxicology
Quasi emulsion spherical crystallization technique based environmentally responsive Tulsion®
(pH dependent) microspheres for colon specific delivery
Ashish Jain
Dr. Hari Singh Gour University, India
Statement of the Problem:
pH-dependent sustained-release Tulsion® microspheres bearing clarithromycin were developed for
colon specific release so that a broad spectrum of diseases can be treated well.
Methodology & Theoretical Orientation:
Clarithromycin bearing Tulsion® microspheres were prepared using quasi-
emulsion solvent diffusion method (spherical crystallization technique) with thermocoat L 30 D-55. Both, clarithromycin
and thermocoat L 30 D-55 were evaluated for
in vitro
toxicity assay against human red blood cells. Ratiometric optimization
of different solvents using phase diagrams was performed on amount of good solvent, bridging liquid, dispersing liquid and
poor solvent.
Findings:
Both, clarithromycin and thermocoat L 30 D-55 were found to be non-hemolytic during
in vitro
toxicity assay
against human red blood cells. The developed microspheres were evaluated for the recovery (67.27±3.3%), average particle
size (52.0±0.46 µm) and encapsulation efficiency (61.0±3.1%). Scanning electron microscopy and transmission electron
microscopy revealed that the microspheres were smooth in surface and spherical in shape, respectively. The drug release study
was conducted at different pH of GIT and it gave a pH dependent release for clarithromycin.
Conclusion & Significance:
The manuscript reported the debut work on thermocoat L 30 D-55 based novel drug delivery
system, the polymer is safe to be used, quasi emulsion spherical crystallization technique is a good technique to prepare
microspheres, the prepared microspheres provides sustain release profile as well as targeting to colon.
ashishsemail@rediffmail.comClin Exp Pharmacol 2017, 7:4 (Suppl)
DOI: 10.4172/2161-1459-C1-020