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Volume 7, Issue 4 (Suppl)
Clin Exp Pharmacol
ISSN: 2161-1459 CPECR, an open access journal
Pharmacology Congress 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
8
th
World Congress on
Pharmacology and Toxicology
Coenzyme Q10-evoked stimulation of nitric oxide-related dilation of the rat aorta
Oleg Medvedev, Larisa Kozaeva, Evgeniya Gorodetskaya
and
Elena I Kalenikova
Lomonosov Moscow State University, Russia
T
his study examined whether coenzyme Q10 can improve NO dependent vasodilatation in the rat aorta after pre-incubation
or intravenous administration. In initial experiments, intact isolated aortic rings were incubated with CoQ10 or L-arginine.
In further experiments, CoQ10 was administered intravenously in anesthetized rats, and then in 2 hours, aorta was isolated. In
both cases, after preliminary preparation the isolated aortic rings were tested for Ach induced NO dependent relaxation. ACh
elicited a concentration dependent relaxation of phenylephrine pre-contracted aortic rings. Relaxant responses to ACh were
markedly potentiated after pre-incubation with CoQ10 or L-arginine. The maximum relaxant responses (%) were significantly
increased from 64.1±5.3 (control) to 89.8±3.0 and 83.6±3.0 (CoQ10 and L-arg, respectively). The pD2 (-lgEC50) value in
control study was 5.81±0.28, after pre-treatment with CoQ10 or L-arg were 7.59±0.16 and 7.26±0.32, respectively. There
was no difference between CoQ10 and L-arginine groups. After intravenous administration, the relaxant responses to ACh
were significantly increased in CoQ10-treated group (94.2±2.0) compared with controls (68.1±4.4). pD2 values were also
different between control and treatment groups (5.79±0.29 vs. 8.14±0.65, respectively). Thus, CoQ10 improved NO mediated
vasodilation in rat aorta as well as substrate for eNOS-L-arginine. Our data show that exogenous intravenously administered
CoQ10 is able to improve rapidly NO dependent vasodilation in rat aorta, likely due to accumulation of CoQ10 in the vessel
wall. Improvement of endothelial function can contribute, at least in part, to beneficial effects of CoQ10 in cardiovascular
diseases associated with endothelial dysfunction.
oleg.omedvedev@gmail.comClin Exp Pharmacol 2017, 7:4 (Suppl)
DOI: 10.4172/2161-1459-C1-020