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.com
Volume 8
Journal of Nutrition & Food Sciences
ISSN: 2155-9600
Nutri-Food Chemistry
&
Euro Obesity 2018
September 13-15, 2018
JOINT EVENT
September 13-15, 2018 | London, UK
14
th
Euro
Obesity and Endocrinology Congress
&
17
th
World Congress on
Nutrition and Food Chemistry
J Nutr Food Sci 2018, Volume 8
DOI: 10.4172/2155-9600-C7-072
Expression profile of adiponectin in young obese Pakistani subjects
Farah Ashfaq
Lahore College for Women University, Pakistan
O
besity in adolescents and young adults has increased significantly in recent years resulting in the development of chronic
diseases. Obesity induces adipocyte dysfunction, secretion of adipokines and activation of macrophages leading to
inflammatory cytokine production. The aim of the present study was to investigate the adiponectin gene expression in young
subjects of different BMI groups. Study subjects included 300 overweight, obese males and females with an age ranging from
17–30 years. 100 comparable control subjects with normal BMI were included. The data was stratified into normal-weight,
overweight, obese I and obese II groups. Anthropometric parameters including age, BMI, waist circumference, WHR, systolic
and diastolic blood pressure were assessed. The metabolic and inflammatory parameters including glucose, insulin, lipid profile,
leptin, adiponectin, resistin, C-reactive protein and interleukin-6 in serum were measured by chemistry analyzer and ELISA.
RNA extraction was done by TRIZOL method and cDNA synthesis was done by using cDNA synthesis kit. The expression of
target gene was compared with GAPDH on real time PCR using gene specific primers. Serum levels of insulin, leptin, resistin,
CRP, IL-6 were significantly higher in overweight and obese subjects as compared to control subjects (p<0.01). Adiponectin
was significantly low in obese groups (p<0.01). In correlation analysis, adiponectin showed a significant inverse relationship
with BMI, WC (r =-.262, p<.008), (r=-.310, p<.002) p<0.01 and with WHR (r=-.199, p=.046) p<0.05, respectively. Adiponectin
was significantly correlated with fasting insulin (r=-.282, p=.004) p<0.01 in obese group. The expression of adiponectin gene
was significantly influenced by obesity.
farah_ashfaq@yahoo.com