nutri-food-chemistry-euro-obesity-2018-posters

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Volume 8

Journal of Nutrition & Food Sciences

ISSN: 2155-9600

Nutri-Food Chemistry

Euro Obesity 2018

September 13-15, 2018

JOINT EVENT

September 13-15, 2018 | London, UK

Euro

Obesity and Endocrinology Congress

World Congress on

Nutrition and Food Chemistry

Metabolomic approaches to analyze principal components in pomegranate juice by HPLC and LC-

MS/MS to evaluate adulteration in the commercial juice

Chan Lee, Jinah An, Joowon Lim, Bogyu Choi

and

Joong Hyuck Auh

Chung Ang University, Republic of South Korea

omegranate is one of the expensive fruit due to its short harvest season. Therefore, adulterated pomegranate juice, mixed

with other chief fruit juice, can lead economic benefit to Food Company. In order to distinguish pure pomegranate juice

with its adulterated juice, the major components of pomegranate juice were analyzed together with grape and peach juices

mainly used for manufacturing the adulterated pomegranate juice. Organic acids, polyphenols and flavonoids were analyzed

using HPLC and LC-MS/MS. Malic acid and citric acid were selected as major organic compounds to evaluate purity of

pomegranate juice and tartaric acid was chosen for grape juice. Commercial pomegranate and peach juice products were

collected in domestic markets and polyphenol and flavonoids were compared after LC-MS/MS metabolomic research. The

difference in major metabolites between pure pomegranate juice groups and other juice groups were clearly found through

principal component analysis (PCA). The OPLS-DA (S-plot and VIP-plot) was used to identify metabolites exhibiting

significant differences between pomegranate and peach and 47 metabolites were identified in this study. Among them, five

flavonoids can be selected as indicators to compare pure pomegranate juice with adulterated pomegranate juice. This study

offers analytical method to evaluate the adulteration of pomegranate juice with other fruit juices.

J Nutr Food Sci 2018, Volume 8

DOI: 10.4172/2155-9600-C7-072