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Notes:
Volume 7, Issue 3 (Suppl)
J Nutr Disorders Ther, an open access journal
ISSN: 2161-0509
Page 67
JOINT EVENT
&
July 27-29, 2017 Rome, Italy
Advances in Natural Medicines Nutraceuticals & Neurocognition
14
th
International Conference on Clinical Nutrition
13
th
International Congress on
Andrographolide activates Keap1/Nrf2/ARE/HO-1 pathway in HT22 cells and suppresses microglial
activation by Aβ42 through Nrf2-related inflammatory response
Won Keun Oh
and
Ji Yeon Seo
Seoul National University, South Korea
T
herapeutic approach of Alzheimer’s disease (AD) has been gradually diversified. We examined the therapeutic and
preventive potential of andrographolide, which is a lactone diterpenoid from
Andrographis paniculata
, and focused on
the Kelch-like ECH-associated protein (Keap1)/nuclear factor (erythroid-derived), (Nrf2)-mediated heme oxygenase (HO)-1-
inducing effects and the inhibitory activity of amyloid beta (Aβ)42-induced microglial activation related to Nrf2 and nuclear
factor κ B (NF-κB)-mediated inflammatory responses. Andrographolide induced the expression and translocation of Nrf2 from
the cytoplasm to the nucleus, thereby activating antioxidant response element (ARE) gene transcription and HO-1 expression
in murine hippocampal HT22 cells. Andrographolide eliminated intracellular Aβ
42
in BV-2 cells and decreased the production
of interleukin (IL)-6, IL-1β, prostaglandin (PG)E
2
and nitric oxide (NO) because of artificial phagocytic Aβ42. It decreased
pNF-κB accumulation in the nucleus and the expression of inducible nitric oxide synthase (i-NOS) and cyclooxygenase
(COX)-II in the microglial BV-2 cell line. In summary, andrographolide activates Nrf2-mediated HO-1 expression and inhibits
Aβ
42
-overexpressed microglial BV-2 cell activation. These results suggested that andrographolide might have the potential for
further examination of the therapeutics of AD.
Biography
Won Keun Oh has acquired his PhD degree in studies about natural product’s chemistry from Korea Advanced Institute of Science and Technology (KAIST). He
has worked as a Visiting Scholar in Baylor College of Medicine, USA (2002-2004). He also worked in Korea Research Institute of Bioscience and Biotechnology
(KRIBB) as a Principle Researcher (2005-2007) and moved to College of Pharmacy in Chosun University as Assistant Professor (2007-2013). He has worked
at College of Pharmacy in Seoul National University as Associate Professor of Pharmacognosy since 2013. He has undertaken more than ten projects of the
Korean Government including Individual Basic Science and Engineering Research Program (2012-2015), the Procurement and Development of Foreign Biological
Resources (2010-2016) and the Korea Bioactive Natural Material Bank (KBNMB, 2012-2017), et al.
wkoh1@snu.ac.krWon Keun Oh et al., J Nutr Disorders Ther 2017, 7:3(Suppl)
DOI: 10.4172/2161-0509-C1-007