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Notes:
Volume 7, Issue 3 (Suppl)
J Nutr Disorders Ther, an open access journal
ISSN: 2161-0509
Page 71
JOINT EVENT
&
July 27-29, 2017 Rome, Italy
Advances in Natural Medicines Nutraceuticals & Neurocognition
14
th
International Conference on Clinical Nutrition
13
th
International Congress on
Protective effect of perilla oil and alpha-linolenic acid on neuronal apoptosis induced by oxidative
stress
Ah Young Lee
1
, Hyun Young Kim
2
, Sanghyun Lee
3
, Myoung Hee Lee
4
and
Eun Ju Cho
1
1
Pusan National University, Republic of Korea
2
Gyeongnam National University of Science and Technology, Republic of Korea
3
Chung-Ang University, Republic of Korea
4
National institute of Crop Science - Rural Development Administration, Republic of Korea
O
xidative stress plays a key role in neuronal cell damage, which is associated with neurodegenerative disease. Recently,
attention has been paid to searching for natural source that can protect neuronal cell damage against oxidative stress.
Although there are evidences that dietary n-3 fatty acid has beneficial effects, it has been focused on fish oil. Alpha-
linolenic acid (ALA) is also promising n-3 fatty acids in vegetable oils with health beneficial roles. Perilla oil (PO) contains
the largest proportion of ALA. However, the study on molecular mechanism between ALA and hydrogen peroxide (H
2
O
2
)-
induced neuronal apoptosis has not been studied yet. The aim of present study is to investigate the neuro-protective effects
of PO and ALA against H
2
O
2
-induced oxidative stress in SH-SY5Y neuronal cells. Our result showed that treatment
with PO and ALA significantly attenuated H
2
O
2
-induced cell death, indicating that PO and ALA potently blocks H
2
O
2
-
induced neuronal apoptosis. Furthermore, cleaved-poly ADP ribose polymerase, cleaved caspase-3 and -9 activations
were decreased in the presence of PO and ALA and H
2
O
2
-mediated down-regulated Bax/Bcl-2 ratio was blocked after
treatment with PO and ALA. Taken together, the protective role of PO from neuronal cell damage is mainly attributed to
ALA. The present study suggests that PO and ALA may play potent therapeutic agents for neurodegenerative disorders
such as Alzheimer’s disease.
Biography
Ah Young Lee is a PhD candidate in Food Science and Nutrition at Pusan National University of Korea, and conducts study under the guidance of Professor
Eun Ju Cho. Her research activities include Bioavailability of Natural Sources in vitro and in vivo system. She has authored or co-authored 18 manuscripts, and
is a fellow funded by Global PhD Fellowship from National Research Foundation. Her laboratory mainly conducts research on development of nutraceuticals
with cognitive improvement using natural sources, and currently interests in mechanisms of amyloid beta metabolism that helps explain neurodegenerative
disease such as Alzheimer’s disease.
lahy1004@hanmail.netAh Young Lee et al., J Nutr Disorders Ther 2017, 7:3(Suppl)
DOI: 10.4172/2161-0509-C1-007