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Volume 7, Issue 6 (Suppl) J Bacteriol Parasito
ISSN: 2155-9597 JBP, an open access journal Microbiology 2016
November 28-29, 2016
November 28-29, 2016 Valencia, Spain
7th World Congress on
Microbiology
Daniel Oliver Connor, J Bacteriol Parasitol 2016, 7:6 (Suppl)
Identification of immunogenic proteins of human pathogenic bacteria utilizing phage display
Daniel Oliver Connor
Fraunhofer Institute for Cell Therapy and Immunology-IZI, Germany
Recent evolutionary development of antibiotic resistances of many human pathogens leads to an inevitable necessity to discover
new ways in diagnostics and treatment of those pathogenic bacteria. Unfortunately, knowledge of suitable antigens and promising
protein candidates is not provided for many pathogens. Therefore, the aim of this study was to identify novel immunogenic proteins
of three different human pathogenic bacteria: Borrelia burgdorferi, Neisseria gonorrhoeae and Neisseria meningitidis. Genomic
phage display libraries of all three pathogens were constructed with 106-107 individual clones and screened for the identification
of immunogenic proteins. Subsequently, corresponding full length proteins were expressed and their immunogenic character
verified by ELISA. 21 potentially immunogenic proteins were identified for N. gonorrhoeae wherefrom six proteins were described
as immunogenic for the first time. The determined immunogenic proteins of and N. meningitidis had been mostly
described in literature but could be verified in this work. Additionally, these results showed that the identification of immunogenic
proteins utilizing phage display is feasible for human pathogenic bacteria and a fast and straightforward approach. The identified
proteins will be further examined for linear epitopes to identify the immunodominant regions. Furthermore, ongoing studies
include the development of recombinant antibodies against the identified proteins. These antibodies will then be used for further
characterization of the identified immunogenic proteins and to investigate their potential as suitable candidates including diagnostics.
Biography
Daniel Oliver Connor has recently submitted his PhD thesis at the University of Potsdam including two first author peer reviewed publications. He has completed
his BSc at the Hochschule Furtwangen University and MSc at the Westfälische Wilhelms-Universität Münster. He has contributed his knowledge and scientific
experience at Merckle Biotech, NMI Tübingen and the Fraunhofer IZI-BB (formerly IBMT) in Potsdam.