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Volume 7, Issue 6 (Suppl) J Bacteriol Parasito
ISSN: 2155-9597 JBP, an open access journal Microbiology 2016
November 28-29, 2016
November 28-29, 2016 Valencia, Spain
7th World Congress on
Microbiology
Lena Danckert, J Bacteriol Parasitol 2016, 7:6 (Suppl)
Infection-like approach to identify immunogenic proteins of Salmonella enteritidis
Lena Danckert
Fraunhofer Institute for Cell Therapy and Immunology IZI, Germany
Salmonella enteritidis is a human food-borne pathogen and one of the most frequently detected serovars of Salmonella. Around
100000 cases of salmonellosis are reported in the European Union annually. After ingestion, the first step of infection is within the
small intestine. The pathogen invades the host and leads to an inflammation within the intestinal epithelium from which the intruder
may disseminate systemically. Bacteria are fast changing microorganisms with the ability to adapt their metabolism in response to
environmental variations. Hence in this study, Salmonella enteritidis is examined under infection-like conditions to reveal the RNA,
which is a snapshot of the bacterial environment. The aim of this study is to identify new immunogenic and virulence-associated
proteins of Salmonella enteritidis. Therefore, the bacterial transcriptome is analyzed through RNA sequencing and cDNA library
screenings. The infection-like approach is based on the invasion of Salmonella enteritidis in the human intestinal CaCo-2 cell line
and the subsequent addition of gentamicin. Infection-like conditions may favor the number of immunorelevant proteins during
immunoscreenings. That implies an advanced method to identify immunogenic proteins. Moreover, using RNA-seq, virulence-
associated factors can be identified by gene expression profiles, thus furthering the understanding of the underlying pathogenicity of
Salmonella, in general and of Salmonella enteritidis, in particular.
Biography
Lena Danckert has completed her MSc at the University of Potsdam and pursuing PhD since in 2013. She is currently working in the Molecular Biology Department
of the Fraunhofer Institute for Cell Therapy and Immunology at the branch Bioanalytics and Bioprocesses in Potsdam, Germany.