microbiology-2016_posters-accepted-abstracts

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conferenceseries

.com

Volume 7, Issue 6 (Suppl)

J Bacteriol Parasito

ISSN: 2155-9597 JBP, an open access journal

Microbiology 2016

November 28-29, 2016

November 28-29, 2016 Valencia, Spain

World Congress on

Microbiology

Wittwer Geraldine et al., J Bacteriol Parasitol 2016, 7:6 (Suppl)

Study of marine actinobacteria diversity from X region de Los Lagos and evaluation of genes PKS/

NRPS as markers of biological activity

Wittwer Geraldine

1, 2

, Camara Beatriz

, Michael Seeger

and

Godoy Rojas Felix

Universidad De Los Lagos, Chile

Universidad Tecnica Federico Santa Maria, Chile

arine bacteria of the phylum actinobacteria have shown to exhibit the ability to produce a wide range of drugs with multiple

biological activities such as antimicrobial, antitumor, antiviral, antiparasitic, insecticides and others. These bacteria are widely

distributed in marine environments, but its greatest diversity is found in sediments and sponges. PCR primers targeted production

of secondary metabolites as Non-ribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) is usual used as molecular

markers of metabolic and biotechnological potential screening of these bacteria. In the present study we isolated 2400 strains of

sponge and sediment samples, from Region de Los Lagos in Chile. The 16S rRNA gene sequencing showed that nearly 20% of these

strains belong to phylum actinobacteria, compound of 22 different genera. The most representative genera found in the samples was

Streptomyces

spp.,

Rhodococcus

spp.,

Nocardiopsis

sp. and

Arthrobacter

spp. To determine the biotechnological potential of these

strains one screening was performed, to detect the presence of PKSI, PKSII, PKSIII and NRPS genes. Result indicates that 80% of the

strains had the presence of one of these genes. And about 30% of the strains obtained had genes two or more of the synthetic routes

discussed. These results demonstrate that marine actinobacterias have broad potential for finding natural compounds with biological

activity and sediment and sponges Los Lagos Region has a wide diversity of marine Actinobacterias.

Biography

Geraldine Wittwer is a Dr(c) in biotechnology of UTFSM and PUCV from Chile. She has 9 years' experience working in enviromental marine Microbiology.

Biochemistry from Austral University.