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.com
Volume 7, Issue 6 (Suppl)
J Bacteriol Parasito
ISSN: 2155-9597 JBP, an open access journal
Microbiology 2016
November 28-29, 2016
November 28-29, 2016 Valencia, Spain
7
th
World Congress on
Microbiology
GolbargMalekhoseini, J Bacteriol Parasitol 2016, 7:6 (Suppl)
http://dx.doi.org/10.4172/2155-9597.C1.026Simultaneous identification of
Mycoplasma gallisepticum
and
Mycoplasma synoviae
by duplex PCR
assay
Golbarg Malekhoseini
Islamic Azad University, Iran
M
ycoplasma gallisepticum
and
Mycoplasma synoviae
have recognized as common respiratory pathogens especially in chickens
causing lots of economical losses in poultry industries. The aim of this study was to develop and validate duplex Polymerase
Chain Reaction (PCR) for simultaneous detection of
M. gallisepticum
and
M
.
synoviae
. A total of 50 samples from tracheas, lungs
and air sacs were taken from commercial broiler chicken farms in Iran. The samples were cultured in PPLO broth supplemented
for
M.
gallisepticum
and
M.
synoviae
isolation and bacteria DNA were extracted by phenol/chloroform extraction method. The
conserved region of
16S rRNA
gene was applied for the detection of
Mycoplasma
genus in 163 bp fragment and
M. gallisepticum
in
183 bp fragment and vlhA gene was also employed for detection of
M.
synoviae
in 350 bp fragment. Hence, duplex PCR amplified
the conserved region of
16S rRNA
and
vlhA
genes which were then applied for detection of
M.
gallisepticum
and
M.
synoviae
. 20
samples in
Mycoplasma
genus and 7 samples in
M. gallisepticum
and
M.
synoviae
were positive in the single PCR whereas in 3 samples
M.
gallisepticum
and
M.
synoviae
were simultaneously positive in the duplex PCR method. The results showed that duplex PCR
was successful to simultaneous identification of
M.
gallisepticum
and
M.
synoviae
and suggested that duplex PCR is more rapid and
inexpensive method than the single PCR for detection of
M. gallisepticum
and
M. synoviae
.
Biography
Golbarg Malekhoseini has completed her MSc in 2011 from University of Qom, Pakistan. Currently, she is an Assistant Professor at Islamic Azad University of Arak
and also working as a Manager of Quality Control in Ice Factory of Arak.
g1_malekhoseini@yahoo.com