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Volume 7, Issue 6 (Suppl) J Bacteriol Parasito
ISSN: 2155-9597 JBP, an open access journal Microbiology 2016
November 28-29, 2016
November 28-29, 2016 Valencia, Spain
7th World Congress on
Microbiology
Roshan Kumar et al., J Bacteriol Parasitol 2016, 7:6 (Suppl)
Comparative genomic analysis reveals environment and habitat specific gene diversity within genus
Novosphingobium
Roshan Kumar and Rup Lal
University of Delhi, India
The genus Novosphingobium is comprised of metabolically versatile bacteria within the family sphingomonadaceae.
Novosphingobium species have been isolated from a wide range of ecological habitats and possess diverse physiological and
biotechnological importance. In this study genomic attributes, phylogenetic relationships, the pan-genome and core genome content
of 27 Novosphingobium strains were compared and analyzed. The study revealed a high level of variation in the genome size, coding
potential and %GC content, suggesting the role of geographical location in shaping the genome of bacteria as they have different
source of isolation. Interestingly, the phylogenetic analysis revealed that the impact of habitat on phylogeny was masked by overall
genomic repertoire. The core genome and pan-genome analysis revealed the overall genomic trend of genus Novosphingobium.
Thereafter, the strains were categorized based on their ecological habitats into four groups i.e., rhizosphere, contaminated soil, marine
and freshwater. Out of 27 Novosphingobium strains, only 19 strains fall in this category. The habitat-based study revealed different
modes of sulfur acquisition and metabolism across the four habitats with the presence of alkane sulfonate (ssuABCD) assimilation
pathway in all the rhizospheric isolates. We also find that the genes/pathway for ectoine biosynthesis was present beyond the marine
habitat, suggesting its relevance beyond the marine habitat. Further, the phage mediated acquisition appears to play a prominent role
in adaptability of the members in their respective habitats. We also find that these Novosphingobium stains codes for numerous mono-
and dioxygenases, responsible for their ability to metabolize several aromatic compounds. The current study provides the genetic
basis for understanding their adaptability into their habitats and their vast potential to degrade a variety of aromatic compounds.
Biography
Roshan Kumar has completed his PhD from University of Delhi, India. His work mostly comprised of comparative genomics, taxonomical studies of strains isolated
from stressed niches such as hexachlorocyclohexane contaminated dumpsites. He has published more than 10 papers in reputed journals and has been serving
as an Assistant Professor at the University of Delhi, India.