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conferenceseries.com
Volume 7, Issue 6 (Suppl) J Bacteriol Parasito
ISSN: 2155-9597 JBP, an open access journal Microbiology 2016
November 28-29, 2016
November 28-29, 2016 Valencia, Spain
7th World Congress on
Microbiology
LuisaMariaMontoya Porras et al., J Bacteriol Parasitol 2016, 7:6 (Suppl)
Analysis of triatomine midgut bacterial diversity, insect vectors of Chagas disease by next generation
sequencing methods (NGS)
Luisa Maria Montoya Porras, Claudia Ximena Moreno-Herrera, Omar Triana-Chavez and Gloria Ester Cadavid-Restrepo
National University of Colombia, Colombia
C hagas disease affects more than 6 million people in Latin America, it is a parasitic disease caused by the protozoan Trypanosoma
cruzi, which is transmitted mainly by bloodsucking insects of the subfamily Triatominae; the study on microbial communities
that inhabit the insect gut is important to understand their role in the parasite transmission and development. The aim of this
work was to evaluate the gut bacterial composition of two triatomine species from Colombia, using high-throughput sequencing
technologies. The insects were collected from housing peridomestic area at Vichada department and wildlife habitat at Magdalena
department then they were identified by conventional taxonomy as: Triatoma maculata and Rhodnius pallescens and their gut were
dissected under aseptic conditions in order to obtain total DNA. After DNA quality confirmation, the sequencing of V4 region from
16S rRNA gene was carried out using the Illumina platform MiSeq. The reads thrown were edited and paired obtaining a size of
250 bp. These sequences were analyzed with the RDP-Classifier software and it showed that 13 bacterial genus are present in both
species, being Burkholderia, Gordonia, Lactococcus and Ralstonia , the most abundant genus. Furthermore, representative genuses
of each species were found. Williamsia and Kocuria were the most common in R . pallescens and the genus Curvibacter, Dietzia and
Pelomonas were only observed in T. maculata. This is the first study of microbiota associated with these triatomine species using
massive sequencing methods. Some of the genus found in this research, have been reported in previous studies of other species of
Chagas disease insect vectors, which may suggest a close association between microbiota and host.
Biography
Luisa Maria Montoya Porras is a Biological Engineer of the National University of Colombia, studying Master of Science in Biotechnology. She has worked in the
research group Microbiodiversity and Bioprospection for four years in microbial diversity of insect crop pests and insect vector of tropical disease. Currently she
is a young Researcher with the “call 706” of the Administrative Department of Science, Technology and Innovation of Colombia (COLCIENCIAS) for the second
consecutive year with the project: Analysis of triatomine midgut bacterial diversity, insect vectors of Chagas disease by next generation sequencing methods (NGS).
Luisa Maria Montoya Porras is a Biological Engineer of the National University of Colombia, studying Master of Science in Biotechnology. She has worked in the
research group Microbiodiversity and Bioprospection for four years in microbial diversity of insect crop pests and insect vector of tropical disease. Currently she
is a young Researcher with the “call 706” of the Administrative Department of Science, Technology and Innovation of Colombia (COLCIENCIAS) for the second
consecutive year with the project: Analysis of triatomine midgut bacterial diversity, insect vectors of Chagas disease by next generation sequencing methods (NGS).