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.com

Volume 7, Issue 6 (Suppl)

J Bacteriol Parasito

ISSN: 2155-9597 JBP, an open access journal

Microbiology 2016

November 28-29, 2016

November 28-29, 2016 Valencia, Spain

World Congress on

Microbiology

Karina Paredes Paliz et al., J Bacteriol Parasitol 2016, 7:6 (Suppl)

http://dx.doi.org/10.4172/2155-9597.C1.026

Design of a bacterial inoculants for phytoremediation of heavy metals in contaminated estuaries

Karina Paredes Paliz

and

Garcia Gonzalez

Universidad de Sevilla, Spain

he aim of our work was the isolation and characterization of bacteria from the rhizosphere of

Spartina maritima

in the

metal contaminated Odiel estuary (Huelva, SW Spain). From 25 strains, 84% were identified as Gram-positive, particularly

Staphylococcus

and

Bacillus

. Gram-negative bacteria were represented by

Pantoea

and

Salmonella

. Salt and heavy metal tolerance,

metal bioabsorption, plant growth promoting (PGP) properties and biofilm formation were investigated in the bacterial collection.

Despite the higher abundance of Gram-positive bacteria, Gram-negative isolates displayed higher tolerance toward metalloids (As,

Cu, Zn and Pb) and greater metal biosorption, as deduced from ICP-OES and SEM-EDX analyses. Besides, they exhibited better

PGP properties, which were retained in the presence of metals and the ability to form biofilms. Gram-negative strains

Pantoea

agglomerans

RSO6 and RSO7, together with Gram-positive

Bacillus aryabhattai

RSO25, were selected for a bacterial consortium

aimed to inoculate

S. maritima

plants in metal polluted estuaries for phytoremediation purposes.

Biography

Karina Paredes Paliz is a Young Researcher from Ecuador who has completed her degree in Biology. She is currently pursuing her PhD in Molecular Biology and

Biomedicine in the Department of Microbiology and Parasitology of the Faculty of Pharmacy, University of Seville, Spain. Her research focuses on environmental

biotechnology, specifically the bioremediation of heavy metals with the use of bacteria and plants. Several of her works focus in the phytoremediation of estuaries

contaminated near to the River Odiel, province of Huelva-Spain.

kparedes@us.es