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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume: 8
June 28-29, 2018 | Alexandria, Egypt
International Pre Conference Workshop on
Microbial Ecology & Eco Systems
Zinc oxide nanoparticales induced histological, histochemical and genotoxicity effects in kidney of
adult male rabbits
2
Tito N Habib,
1
Hekmat O Abdel-Aziz
and
3
Eman E Ragab
1
Assistant Prof. of Histology, Faculty of Medicine, Sohag University
2
Professor of Genetics & Molecular Biology, Head of Molecular Genetics' Lab, Zoology department, Faculty of Science, Sohag University
3
Lecturer of Histology, Faculty of Medicine, Sohag University
O
ur aim was to study the histological, histochemical and genotoxic effects of ZnO NPs on the kidney of adult male rabbits.
Fifteen adult male rabbits were divided equally into three groups. Group I was the control group; group II was the low dose
treated group, in which rabbits were intraperitonial injected with ZnO NPs (100 mg/kg/day) for 14 days; and in group III was
the high dose treated group, rabbits were injected intra peritonially with ZnONPs (250 mg/kg/day) for 14 day. At the end of the
experiment, specimens from the kidney were taken and stained by H&E, PAS and Masson trichome stains. Also bone marrows
were isolated for flow cytometry to study genotoxicy. ZnO NPs was nephrotoxic and led to prominent histopathological
changes in the kidney. There were destructions of the renal tubules, in form of loss of brush border, vacuolation of cytoplasm
and intratubular protein depositions. Also there was interstitial infiltration of inflammatory cells. The renal corpuscles were
dilated and congested. There was increase in apoptotic cell rate of bone marrow samples and showed greater and distinct
fragmentation or shearing DNA. ZnO NPs had cytotoxic and genotoxic effects in the kidney. It increased apoptotic rate of the
bone marrow cells and fragmentation of DNA. ROS may be the main cause of cytotoxic effects. ZnO NPs was toxic in dose
dependent manner.
Keywords:
apoptosis, cell cycle, DNA fragmentation, Histology, Histochemistry, genotoxicity, Zinc oxide.
Biography
Tito N. Habib is currently is the head of molecular genetics' Lab., Zoology department, Faculty of Science, Sohag University, since 2015 till 2018, and a professor
of Genetics & molecular Biology. He got his PhD from Texas A& M University, in 1999 as a visiting scholar in the department of Wildlife and Fisheries Science,
Bryan-College Station, Texas, USA, in the field of Molecular Genetics. He followed his promotion as assistant professor of molecular Genetics, Zoology department,
Faculty of Science, Sohag University, Egypt, (2007-2011), and Head of Biology Department, Faculty of Science, Balgurashi, Albaha University, KSA (2011-2015).
He served as HEEPF project council member (B-035-P1) for the development of Genetics' courses, Faculty of Science, Sohag University, Egypt. He was a main
advisor for 2 PhD, and 2 Master Thesis in Medical Genetics topics, Faculty of Medicine, Sohag University. He served as a Head of the Egyptian Syndicate of
Scientific professions (Sohag-branch) from December, 2017 till February, 2020. He works as a reviewer for journal of Clinical Pathology and Forensic Medicine,
Chronicle Journal of Cancer Science. He got an invitation from 7th International Conference on Biomedical Engineering and Biotechnology (ICBEB 2018) which
will be hold in October 17th - 20th, 2018, Nanjing, China.
titohabib99@yahoo.comTito N. Habib et al., J Ecosys Ecograph 2018, Volume: 8
DOI: 10.4172/2157-7625-C2-036