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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume: 8
June 28-29, 2018 | Alexandria, Egypt
International Pre Conference Workshop on
Microbial Ecology & Eco Systems
Transgenic approach towards the enhancement of cyanidase production by
Chlamydomonas
sp
algae
Noha El-Sharkawi, Y M El-Ayouty, Rashad Kebeish
and
M Gomaa
Plant Biotechnology Lab., Faculty of Science, Zagazig University
C
yanide is a nitrile, an organic compound that contains a triple-bonded carbon and nitrogen functional group. Most of these
compounds are highly toxic, carcinogenic, and mutagenic. The toxicity of cyanide is quite high due to its ability to poison
the respiratory system by inhibiting the final transport electrons from cytochrome C oxidase to oxygen, and finally preventing
production of ATP. Although chemical and physical treatments provide more rapid detoxification and are less susceptible to
environmental upsets biological alternatives are more economical and good for ecological balance. The aim of the present study
is to detoxify the toxicity of cyanide to algae through the transformation of some related gene (cyanidase) to
Chlamydomonas
reinhardtii
. Via
Agrobacterium tumefaciens
.. Restriction enzymes, gel-electrophoresis and RT-PCR techniques indicate that
Chlamydomonas reinhardtii is transgenic and has well overexpression of cyanidase and can be used for bioremediation of
nitrile compounds.
Keywords:
Chlamydomonas, Agrobacterium
, P. stutzeri AK61, cyanidase gene
Biography
Noha El-sharkawi is currently a biology teacher at el Awael private school, Zagazig, Egypt. She has pre-MSc in Botany (Microbiology) 2012. She has experience
in scientific research. She has a good knowledge in Writing Scientific Thesis. She has a good experience in computer science. She has MCPD, MCTS from IBM
training courses,courses of Human Recourses (Dale Carnegie Training), Toefel in English.
sunsetno2006@yahoo.comNoha El-Sharkawi et al., J Ecosys Ecograph 2018, Volume: 8
DOI: 10.4172/2157-7625-C2-036