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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume: 8
June 28-29, 2018 | Alexandria, Egypt
International Pre Conference Workshop on
Microbial Ecology & Eco Systems
Biosynthesis of silver nanoparticles using
Aspergillus oryzae
and its extracellular protein profile
Marwa R Obiedallah, Mohamed B Aboul-Nasr
and
Sabah S Mohamed
Faculty of Science, University of Sohag, Egypt
T
he biological generation of silver nanoparticles (AgNP) is a straight forward process. However, the mechanism by which
this process takes place is unclear. We demonstrate the synthesis of AgNPs using fungal filtrate of
Aspergillus oryzae
.
A characteristic peak of AgNP formation was detected at 435 nm. Eight most prominent extracellular protein bands were
identified using MALDI/TOF Mass spectrometry. Here, we suggest that proteinaceous molecules such as some amino acids,
play an essential role in converting AgNO
3
into AgNP. Moreover, this is the first report suggesting that AgNP are stabilized
by the chelating agent, 5-hydroxy-2-hydroxymethyl-1,4-pyrone (kojic acid). Our findings suggest that this species would be
useful for large scale AgNP production.
Keywords:
Aspergillus oryzae
, MALDI-TOF, Silver nanoparticles
Biography
Marwa R. Obiedallah is currently an assistant lecturer of Microbiology at Faculty of Science, University of Sohag, Egypt. She has MSc in mycotoxins (2011), and
gained wide experience in fungal natural products and mycotoxins during her study. She was offered a scholarship from the Egyptian cultural affairs and missions
sector for her PhD project at the at School of Biological Sciences, University of Reading, UK, where she had the opportunity to improve her skills and experience.
She is a postgraduate member at the British Mycological Society (2017-2019). Her research interests now is focusing on nanotechnology, where she is paying
attention for the mechanism by which fungal species can generate nanoparticles of their metal salts. She believes that he findings will direct future researches for
proteome studies of promising fungal isolates.
m.r.a.obiedallah@gmail.comMarwa R Obiedallah et al., J Ecosys Ecograph 2018, Volume: 8
DOI: 10.4172/2157-7625-C2-036