microbial-ecology-2018-posters-abstracts

8 / 11

Page 43
Notes:
Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume: 8
June 28-29, 2018 | Alexandria, Egypt
International Pre Conference Workshop on
Microbial Ecology & Eco Systems
Mycogenesis of silver nanoparticles using selected strains of opportunistic fungi and their efficacy
against asergillosis causal agents
Alaa A Yasien, M Bassam, Aboul-Nasr and Sabah S Mohamed
Faculty of Science, University of Sohag, Egypt
Four strains of opportunistic fungi isolated from aspergillosis suspected patients at Assiut university chest department, proved to
possess high enzymatic ability, were tested for silver nanoparticles producing ability. Aspergillus niger EN2 KY6095, A. flavus EN1
KY609, A. terrus EN3 MF 852635 and A. fumigatus filtrates were challenged with 1mM silver nitrate aqueous solution. C EN1 KY609,
A. terrus EN3 MF 852635 and A. fumigatus proved to produce silver nanoparticles, whereas, A niger EN2 KY6095 was not able to
induce AgNP. The process was fast and stable in the case of A. terrus and A. flavus. UV-visible spectrum peaks were shown at 445 and
430, respectively; corresponding with the plasmon absorbance of AgNP. TEM micrographs showed well dispersed AgNPs ranging
of 50-30 nm. Digital Laser Scattering (DLS), Fourier-transform infrared spectroscopy (FTIR) and X-Ray Diffraction confirmed the
production of AgNPs by A. flavus and A. terrus that showed high and stable production of AgNPs. Silver nanoparticles has an
antibacterial effect on different bacterial strains. Its efficacy against gram-negative bacteria is higher than Gram positive bacteria.
Antifungal assay of AgNPs showed significant advantages over conventional antifungal compounds.
Keywords: Silver nanoparticles, opportunistic fungi, A. terrus, A. flavus.
Biography
Alaa A Yasien is currently a Master student at Faculty of Science, University of Sohag, Egypt. She has in microbiology and chemistry (2011), and applied
microbiology diploma (2012) from University of Sohag, Faculty of Science, Sohag, Egypt. Through her study, she developed interest in studying the role of fungi in
the field of nanotechnology. Her research is based on how nanoparticles are produced in a more stable and effective biological way on pathogenic microorganisms.
Alaa A Yasien et al., J Ecosys Ecograph 2018, Volume: 8
DOI: 10.4172/2157-7625-C2-036