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Journal of Ecosystem & Ecography | ISSN: 2157-7625 | Volume: 8
June 28-29, 2018 | Alexandria, Egypt
International Pre Conference Workshop on
Microbial Ecology & Eco Systems
Antitumor effect of scorpion venom peptides
in vivo
of male rabbit and
in vitro
of DU145 cells of
prostate cancer model
2
Nadia S Mahrous, Hemely A Hassan, Fayza M Ali
and
1
Tito N Habib
1
Molecular Genetics’ Lab, Zoology Dept., Faculty of science, Sohag University
2
Cytogenetic Lab, Zoology Dept., Faculty of Science, (Qena) South Valley University, Egypt
T
he modern approach used to characterize various compounds from animal venoms, using advanced proteomic and
genomic tools, has been denominated “venomics”. Venoms from various scorpions have been reported to prevent
propagation of different cell lines such as prostate cancer (DU-145), human leukemia and neuroblastoma. In the present study,
antitumor effect of scorpion venom was detected
in vivo
of male rabbits and
in vitro
of PC-3 cell line using cell cycle profiling
analysis, DNA fragmentation assay, and genetic and epigenetic variations by ELISA kits. The results showed that apoptosis
was maximum at pre-G1, and cell growth arrest at G1 phase in group IV. Venom differentially up regulated gene expression
of P53, BAX, BCL-2. DNA showed greater and distinct fragmentation
in vivo
and
in vitro
of prostate cancer (PC) than venom
treated groups. From the previous result we have concluded that
L. quinquestriatus
’ scorpion venom induced apoptosis and
differentially modulated the expression of tumour suppressor genes and concomitantly repressing the expression of oncogenes
in vivo
of induced male rabbits with PC and
in vitro
of PC-3cell line.
Keywords:
antitumor, apoptosis, cell cycle, DNA fragmentation, prostate cancer, scorpion venom, tumor suppressor gene.
Biography
Nadia S Mahrous is currently an assistant lecturer at Molecular genetics' Lab, Faculty of Science, (Qena) South Valley University, Egypt. She has MSc in
Parasitology (2012), Faculty of Science, Egypt. She has got an experience in teaching practical section of veterinary genetic and genetic engineering in college of
Veterinary Medicine (2013-2018), Sohag University. She participated in different molecular biology and cytogenetics' techniques. She is a member of the Egyptian
Syndicate of Scientific professions.
samirnadia89@gmail.comNadia S Mahrous et al., J Ecosys Ecograph 2018, Volume: 8
DOI: 10.4172/2157-7625-C2-036