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conferenceseries
.com
Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
Analysis of bloodmicrobiome by highly sensitive 16Smetagenomic sequencing:Anew tool for diagnosis
Benjamin Lelouvier
Vaiomer, France
D
iagnosis and treatment of bloodstream infection (BSI) will greatly benefit from sensitive and exhaustive molecular
methods to detect bacterial DNA in blood, such as quantitative PCR (qPCR) and metagenomics sequencing. Such
approaches are already studied with the aim of reducing the turnaround time and increasing the sensitivity of the microbiota
detection in suspected BSI. However, this type of molecular diagnosis is greatly complicated by the presence of human DNA
and PCR inhibitors in blood, as well as bacterial DNA contaminants present in the environment, reagents and consumables,
which dramatically hamper the signal to noise ratio of qPCR and sequencing pipelines. In the course of our investigations into
the role of tissue microbiota in cardiometabolic diseases we developed specific optimized pipelines of qPCR and 16S targeted
metagenomic sequencing to analyze blood bacterial DNA, despite the technical difficulties associated with this sample type.
Using these molecular tools we have demonstrated the existence of a highly diversified blood micro biome in healthy human
donors and shown the association between changes in the blood microbiome and liver fibrosis in obese patients. These assays
were primarily designed to analyze bacterial DNA in blood and tissue of healthy donors and patients with no infectious
disease, and therefore their signal to noise ratios are high and they are also capable of detecting BSI in patients with high
sensitivity and at early stages of infection.
Biography
Benjamin Lelouvier received his PhD in Cellular and Molecular Neurobiology from the University Pierre et Marie Curie, Paris VI, France, in 2007. After a Postdoctoral
Fellowship at the National Institutes of Health (USA), he joined Vaiomer in 2012. As cellular and molecular biology Group Leader and Head of biomarkers discovery,
he developed with his group the molecular tools (16S qPCR and 16S metagenomics sequencing) to study specifically the blood and tissue microbiomes, before
becoming Chief Scientific Officer of Vaiomer in 2016. The study of tissue and blood microbiota allows Vaiomer to link intestinal dysbiosis and tissular inflammation
for the development of biomarkers and therapeutics in the fields of cardiometabolic diseases, neurodegenerative disorders and chronic infection
benjamin.lelouvier@vaiomer.comBenjamin Lelouvier, J Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-026