8 / 22
Page 35
Notes:
conferenceseries
.com
Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
Baofukang suppository promotes the repair of vaginal epithelial cells in response to
Candida albicans
Ting Li, Xiaoxi Niu, Xu Zhang, Suxia Wang
and
Zhaohui Liu
Peking University First Hospital, China
Statement of the Problem:
Vulvovaginal candidiasis (VVC) is an opportunistic fungal infection predominantly caused
by
Candida albicans
affecting a significant number of women of reproductive age. The Chinese medicine, the Baofukang
suppository is widely used in the clinic for its antimicrobial activity and is therefore of great interest as a potential antifungal
drug for the prevention of VVC.
Methodology & Theoretical Orientation:
We evaluated the cytotoxic activity of the Baofukang suppository using the VK2/
E6E7 vaginal epithelial cell (VEC) line. An ELISA analysis was made to evaluate three kinds of cytokines (Th1, Th2 and Th17
types) and non-B IgG in the supernatants. SEM was conducted to observe ultrastructural changes of VECs.
Findings:
When treated with the Baofukang suppository, all of the immunocompetent cytokines and chemokines (e.g., IL-
2, IL-4, IL-6, IL-8, and IL-17) by infected VK2/E6E7 cells was statistically up-regulated (P<0.05), except IL-4 (11.70±1.82
vs. 14.88±4.72, P=0.343) compared to the infected control cells. The secretion of non-B IgG also exhibited the same trend.
Our scanning electron microscopy results revealed that C. albicans can invade VECs by both induced endocytosis and active
penetration. The Baofukang suppository could effectively inhibit the adhesion, hyphal formation, and proliferation, as well as
notably restore the vaginal epithelial cell morphology, viability, and enhance the local immune function of the VECs.
Conclusion&Significance:
These preliminary results suggest promising antimicrobial properties of the Baofukang suppository,
which may be efficacious as an antifungal therapy candidate via up-regulating Th1 cellular immunity, the Th17-axis of the
innate immune response, and the secretion of vaginal epithelial-derived IgG. These combined effects collectively restore the
immune function of the infected VECs against
Candida albicans
in vitro
.
Biography
Ting Li has her expertise in female genital tract infection diseases.
610391330@qq.comTing Li et al., J Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-026