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conferenceseries
.com
Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
Isolation and characterization of bacterial species frompatients with dental caries and caries-free subjects
Hamoud Khalid Alshaya, Khalid Sukhail G Alshammari, Saleh Ahmed Alogla, Carlo L Nazareno, Mohammad Abdulaziz Saud AlJameel
and
Ashfaque
Hossain
University of Hail, Saudi Arabia
Background:
The oral cavity harbours a large number of bacterial species as normal flora existing as biofilm. Dental disease
such as dental caries results when there is a shift in the balance of bacteria towards pathogenic species within these biofilms.
Objective:
The objective of this study was to isolation, identification and characterization of oral bacterial species of patients
with dental caries and caries-free healthy control subjects.
Materials & Methods:
A standard bacteriological procedures were followed in the isolation of bacteria. The identification of
bacteria was carried out using Matrix-Associated Laser Desorption Ionisation–Time of Flight–Mass Spectrometry (MALDI–
TOF–MS) (Bruker MALDI Biotyper system). The characterization of bacteria involved in the determination of biofilm forming
potential and assessment of synergistic antimicrobial action of manuka honey and gentamicin against the oral species.
Results:
A total of 13 bacterial species were isolated from 35 orals samples (10 from patients with dental caries); of which 7
bacterial species have been isolated for the first time in Saudi Arabia. The Streptococcus spp. exhibited varied biofilm-forming
potential and response to synergistic antimicrobial activity of manuka honey and gentamicin.
Conclusion:
The isolation of 7 bacterial species for the first time from dental caries and caries-free subjects in Saudi Arabia
warrants a larger prevalence study involving molecular and phenotypic tests to assess their role in health and disease in Saudi
population.
Biography
Hamoud Khalid Alshaya is a student of Medical College in the University of Hail.
idrhamood@gmail.comHamoud Khalid Alshaya et al., J Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-026