infection-control-microbial-pathogenesis-2018-posters

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Journal of Infectious Diseases & Therapy |ISSN: 2332-0877 | Volume 6

June 25-26, 2018 | Vancouver, Canada

International Conference on

Infection, Disease Control and Prevention

Microbial Pathogenesis & Infectious Diseases

Reverse Antibiotic-Resistance in ESBL

E. coli

using CRISPR technique and programmed bacteriophages

Zhabiz Golkar

Voorhees College, Denmark

Antimicrobial resistance of pathogens such as

E. coli

is a growing concern to the health care system. This increased concern of

pathogen resistance to current therapies is encouraging the development of new antimicrobial strategies, and is reviving interest in

traditional bacteriophage applications. Although therapeutic and prophylactic application of lytic phages has distinct advantages.

over conventional medical interventions, bacteria have evolved multiple defense barriers to interfere with nearly every step of phage

life cycles. Phages counteract this selection pressure by evolving their genomes to evade bacterial resistance. The antagonistim

between bacteria and rapidly mutating viruses promotes the evolution and propagation of phageresistance mechanisms in bacteria.

An adaptive microbial immune system, known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) provides for

acquired immunity against viruses and plasmids. Unlike the restriction–modification anti-phage barrier that cleaves any foreign DNA

lacking a protective methyl-tag in the target site, the CRISPR–Cas systems are invader-specific, adaptive, and heritable. In this study,

we use bacteriophages for delivering a programmable DNA nuclease, CRISPR-associated (Cas), to reverse antibiotic resistance and

to eliminate the transfer of resistance between strains. This novel approach combines CRISPR-Cas delivery with lysogen, lytic phage

selection of antibiotic-sensitized bacteria. The strategy uses phages in a unique way that overcomes many of the hurdles encountered

by phage therapy, and, therefore, may reduce the prevalence of antibiotic-resistant bacteria in designated facilities that may have

concern for MDR strains.

J Infect Dis Ther 2018, Volume 6

DOI: 10.4172/2332-0877-C2-042