40 / 47
Page 100
Notes:
conferenceseries
.com
Volume 6
Journal of Infectious Diseases and Therapy
ISSN: 2332-0877
Infection Congress 2018
March 01-02, 2018
March 01-02, 2018 Berlin, Germany
5
th
International Congress on
INFECTIOUS DISEASES
Emerging drug resistance
Salmonella
strains from chicken meat
Ghoneim Nahed
and
Kadry Mona
Cairo University, Egypt
T
his work was carried out to study the occurrence of salmonella infections in chicken and human suffering from
gastroenteritis in Egypt. A total of 349 chicken meat and 40 stool specimens of children were collected, samples were
subjected to bacteriological examination and identified biochemically as salmonella then subjected to serological identification,
also were tested for their antibiotic susceptibility by disc diffusion method. Also, genotyping by PCR to detect salmonella
enterotoxin gene
(stn)
and their expression by infant mouse assay. The study revealed that 14 (4.01%) and two (5%) were
positive for Salmonella species in chicken meat and children respectively and serological identification were
(Salmonella
infantis, Salmonella typhi, Salmonella kentucky, Salmonella rubislaw, Salmonella poona, Salmonella typhimurium, Salmonella
virginia, Salmonella enteritidis and Salmonella montevideo) and (Salmonella kentucky and Salmonella enteritidis)
in chicken
meat and children isolates respectively. Disc diffusion method showed that three (21.4%) in chicken meat isolates and two
(100%) in children isolates were multidrug resistant in which S. Kentucky have resistance to ciprofloxacin, the drug of choice
for treating salmonellosis in children. Also, genotyping showed that nine (64.28%) and two (100%) isolates confirmed to be
enterotoxigenic strains in chicken meat and children respectively and this (stn) gene have been expressed (100%) by infant
mouse assay. Sequencing and phylogentic tree of four studied isolates resulted in assessment of the relations between different
isolation sources. Special attention must be paid to antibiotics that are used exclusively in poultry farms, appropriate measures
must be taken to control the spread of resistant bacteria to human
.
vet_moony@yahoo.comJ Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C1-039