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Volume 6
Journal of Infectious Diseases and Therapy
ISSN: 2332-0877
Infection Congress 2018
March 01-02, 2018
March 01-02, 2018 Berlin, Germany
5
th
International Congress on
INFECTIOUS DISEASES
Quantitative PCR system for detection of clinically relevant CMV infections in patients with
inflammatory bowel disease
Nils Wethkamp
Head of Molecular Diagnostics, Germany
S
everal lines of evidence indicate that cytomegalovirus infection can be substantially associated with onset of inflammatory
bowel disease, especially in patients who are refractory to immunosuppressive treatment. As cytomegalovirus is widely
spread in the population a quantitative detection system was generated which is suitable to differentiate clinically relevant
cytomegalovirus infection of the intestine from common latent cytomegalovirus. By using a quantitative real-time PCR
approach, cytomegalovirus viral load was evaluated in formalin fixed and paraffin embedded colon biopsy samples of 136
patients diagnosed with inflammatory bowel disease. Besides initial cytomegalovirus testing, the PCR system was also used
to monitor therapy response after antiviral treatment. Cytomegalovirus DNA was detected in 27% patients with varying viral
loads. Thereof, 13 patients (35%) received an antiviral treatment with 12 of them going into remission (92%). Later, five patients
displayed a relapse and three patients who agreed to restart antiviral treatment again showed positive therapy response. A
retrospective comparison of viral loads with antiviral therapy response revealed a threshold of 600 cytomegalovirus copies/105
cells as indicative for clinically relevant infection. Interestingly, we found that sensitivity of cytomegalovirus detection by
immunohistochemistry was insufficient to reliably identify antiviral therapy responders. In summary, quantitative real-time
PCR using formalin fixed biopsy samples is suitable for detection of cytomegalovirus infection in tissue samples of patients
with inflammatory bowel disease. Moreover, it allows the definition of a viral load threshold, predictive for clinical relevance
concerning antiviral therapy response
.
wethkamp@pathologie-vechta.deJ Infect Dis Ther 2018, Volume 6
DOI: 10.4172/2332-0877-C1-039