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conferenceseries
.com
Volume 5, Issue 2 (Suppl)
J Infect Dis Ther 2017
ISSN: 2332-0877, JIDT an open access journal
Infection Congress 2017
May 11-12, 2017
May 11-12, 2017 Barcelona, Spain
4
th
International Congress on
Infectious Diseases
J Infect Dis Ther 2017, 5:2 (Suppl)
http://dx.doi.org/10.4172/2332-0877-C1-024The immunologic effect of hemoperfusion with polymyxin B in trauma-induced swine shock model
Do Wan Kim, Gwan Sic Kim
and
In Seok Jeong
Chonnam National University, South Korea
Introduction & Aim:
Hemoperfusion with adsorption filter is considered to potentially improve the clinical outcomes in septic
patients. However, little is known about the effect of anti-inflammation and immune-regulation by this device in traumatic field. The
aim of this study is to evaluate the efficacy on early application of adsorption filter in swine trauma model.
Method:
Blunt thoracic injury and shock was experimentally made in 10 pigs. Traumatic shock were treated with goal direct therapy
based resuscitation (control group, n=5) or plus hemoperfusion with polymyxin–B adsorption filter (HP group, n=5). Hemoperfusion
was started at hour 1 after induction of traumatic shock. Blood samples were taken from the animals at baseline, 1 and 7 hours after
shock. For anti-inflammatory evaluation, cytokines such as TNF-α, IL-6 were measured by ELISA method and for immunologic
analysis, immune cell activities (T lymphocyte subsets, monocyte and natural killer cell) were evaluated with flow cytometry analyses
(CD3, CD4, CD8, CD14 and CD56).
Results
: Median body weight was 33.1 kg (31.7-38.4 kg). Cytokine study demonstrated that TNF-α and IL-6 were reduced in HP group
after 6 hours of hemoperfusion. However, a progressive increase was detected in control group. In activities of T cell lymphocytes
subsets (CD3, CD4 and CD8), there was no significant difference between two groups; however, the activities of monocyte (CD 14)
and natural killer cell (CD56) were attenuated at 6 hour in HP group, compared to the control group (p<0.05).
Conclusions:
We suggest that the hemoperfusion with adsorption filter should have a positive anti-inflammatory effect in early phase
of traumatic injury. On the other hand, a negative immune-modulation effect might exist by alleviation of innate immune cell activity.
However, further investigation will be needed.
maskjoa@naver.com`Characterization bioactive constituents of essential oil of
Lavandula angustifolia
Mill: Other treatment
against human infections
Hadjer Boudjemaa
and
Rachida Allem
Université Hassiba Benbouali de Chlef, Algeria
Background:
Urinary and vaginal infectious diseases reached at high rate, issues of emerging and re-emerging infections due to drug-
resistant bacteria becomes a serious danger to human health, medicinal plants constitute a reservoir of bioactive molecules that can
kill multidrug resistant microbial infection. In the context of this research, study was intended to characterize molecules bioactive of
essential oil and its potentials against urinary and vaginal infections.
Methods:
The potential antimicrobial was characterized
in vitro
against 12 reference and clinicalmulti drug resistant strain encountered
in clinical laboratory and the microbiology laboratory of Saidal Medea, Algeria. The minimum inhibitory concentrations (MIC) and
the minimum microbicidal concentration (MMC) were determined by disc diffusion assay and agar dilution assay; GC/MS and CPG
were utilized to detect the content of bioactive molecule of essential oil.
Results:
All strains was appeared sensitive to essential oil of
Lavandula angustifolia
Mill, minimum concentration value was 0.001
v/v can inhibit growth and kill
E. coli
ATCC1536 and
Citrobacter braakii
and
Micrococcus luteus
and
Bacillus subtilis
ATCC6133,
C. albicans
and
C. albicans
ATCC1231. Essential oil presented a good potential biostatic/biocidal.
Lavandula angustofolai
Mill was
extremely higher against almost all microbes tested. The composition of bioactive molecule of essential oil was characterized as 45
components made up principally of linalyl anthranilate (31.92%) and linalool (25.63%).
Conclusion:
Lavender essential oil possesses strong potential microbicide against the bacterial and fungal human pathogens strains,
it can consider as an alternative treatments.
boudja.hadjira@yahoo.fr